Zika Virus Methods and Protocols /

書目詳細資料
企業作者: SpringerLink (Online service)
其他作者: Kobinger, Gary (Editor), Racine, Trina (Editor)
總結:XII, 290 p. 57 illus., 42 illus. in color.
text
語言:英语
出版: New York, NY : Springer US : Imprint: Humana, 2020.
版:1st ed. 2020.
叢編:Methods in Molecular Biology, 2142
主題:
在線閱讀:https://doi.org/10.1007/978-1-0716-0581-3
格式: 電子 圖書
書本目錄:
  • Zika Virus: A Brief History and Review of its Pathogenesis Rediscovered
  • Zika Virus Isolation, Purification, and Titration
  • Evaluating Zika Virus Pathogenesis in Immunocompromised Mice
  • Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice
  • A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies
  • Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test
  • Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation
  • Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells
  • Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA
  • Zika Virus Peptide ELISA (ZIKV-NS2B-concat ELISA) for Detection of IgG Antibodies to Zika Virus Infection
  • Zika Virus Amplification using Strand Displacement Isothermal Method and Sequencing using Nanopore Technology
  • Detection of Zika Virus using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP)
  • Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus
  • Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA
  • A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response
  • Isolation and Detection of Zika Virus-Infected Rhesus Macaques Lymph Node Cells and Splenocytes
  • Discovery of Zika Virus Dependency and Restriction Factors using Flow-Based Arrayed CRISPR Screening for Identification of Targets (FACS-IT)
  • Zika Virus Targets Multiple Tissues and Cell Types During the First Trimester of Pregnancy
  • Use of Primary Human Fetal Astrocytes and Tissue Explants as Ex Vivo Models to Study Zika Virus Infection of the Developing Brain
  • Isolation of Monoclonal Antibodies from ZikaVirus-Infected Patient Samples.