Characterization of Plant Viruses Methods and Protocols /

书目详细资料
Main Authors:	Bhat, Alangar Ishwara (Author), Rao, Govind Pratap (Author)
企业作者:	SpringerLink (Online service)
总结:	XXI, 552 p. 161 illus., 99 illus. in color. text
语言:	英语
出版:	New York, NY : Springer US : Imprint: Humana, 2020.
版:	1st ed. 2020.
丛编:	Springer Protocols Handbooks,
主题:	Plant diseases. Virology. Plant genetics. Plant physiology. Plants > Development. Plant Pathology. Plant Genetics. Plant Physiology. Plant Development.
在线阅读:	https://doi.org/10.1007/978-1-0716-0334-5
格式:	电子电子书

书本目录:

Chapter 1. Glasshouse for maintenance of virus and insect culture
Chapter 2. Symptoms of virus infected plants
Chapter 3. Isolation and diagnosis of virus through indicator hosts
Chapter 4. Host range of viruses
Chapter 5. Physico-chemical properties of virus in crude sap
Chapter 6. Mechanical sap transmission
Chapter 7. Transmission through grafting and budding
Chapter 8. Transmission through dodder
Chapter 9. Virus transmission through Pollen
Chapter 10. Transmission through seeds
Chapter 11. Transmission of viruses by aphids
Chapter 12. Transmission of viruses by leafhoppers
Chapter 13. Transmission of virusesby whiteflies
Chapter 14. Transmission of virusesby thrips
Chapter 15. Transmission of viruses through mealybugs
Chapter 16. Transmission of viruses through beetles
Chapter 17. Transmission of viruses through mites
Chapter 18. Transmission of viruses through fungi
Chapter 19. Transmission of viruses through nematodes
Chapter 20. Storage andpreservation of plant virus cultures
Chapter 21. Purification of Plant Viruses
Chapter 22. Ultraviolet absorption spectra of purified virus preparation
Chapter 23. Electron microscopy and Utramicrotomy
Chapter 24. Determination of coat protein molecular weight of viruses
Chapter 25. Isolation of nucleic acid from purified virus and determination of its nature
Chapter 26. Agarose gel electrophoresis for nucleic acids
chapter 27. In vitro expression of viral coat protein in prokaryotic system and its purification
Chapter 28. Production of Polyclonal Antiserum
Chapter 29. Production of monoclonal antibody
chapter 30. Serological Tests
Chapter 31. Isolation of total DNA from plants
Chapter 32. Isolation of total RNA from plants
Chapter 33. Isolation of double stranded (ds) RNA from virus infected plants
Chapter 34. Dot-blot hybridization technique
Chapter 35. Polymerase chain reaction
Chapter 36. Real-time polymerase chain reaction
Chapter 37. DNA Microarray for detection of plant viruses
Chapter 38. Loop-mediated isothermal amplification (LAMP)
Chapter 39. Rolling circle amplification (RCA)
Chapter 40. Recombinase polymerase amplification
Chapter 41. Next generation sequencing for diagnosis of viruses
Chapter 42. Cloning of PCR Product
Chapter 43. cDNA synthesis and cloning
Chapter 44. DNA sequencing
Chapter 45. Sequence analysis and phylogenetic studies
Chapter 46. Development of infectious clone of virus
Chapter 47. Virus elimination by meristem-tip culture
Chapter 48. Virus elimination through somatic embryogenesis
Chapter 49. Production of virus-resistant plants through transgenic approaches
Chapter 50. Production of virus-resistant plants through CRISPR-Cas technology.

Characterization of Plant Viruses Methods and Protocols /

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