Osteogenic Potential and Long-Term Enzymatic Biodegradation of PHB-based Scaffolds with Composite Magnetic Nanofillers in a Magnetic Field
| Parent link: | ACS Applied Materials and Interfaces.— .— Washington: American Chemical Society Vol. 16, iss. 42.— 2024.— P. 56555–56579 |
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| Andere auteurs: | , , , , , , , , , , , , , , , , , , , |
| Samenvatting: | Title screen Millions of people worldwide suffer from musculoskeletal damage, thus using the largest proportion of rehabilitation services. The limited self-regenerative capacity of bone and cartilage tissues necessitates the development of functional biomaterials. Magnetoactive materials are a promising solution due to clinical safety and deep tissue penetration of magnetic fields (MFs) without attenuation and tissue heating. Herein, electrospun microfibrous scaffolds were developed based on piezoelectric poly(3-hydroxybutyrate) (PHB) and composite magnetic nanofillers [magnetite with graphene oxide (GO) or reduced GO]. The scaffolds’ morphology, structure, mechanical properties, surface potential, and piezoelectric response were systematically investigated. Furthermore, a complex mechanism of enzymatic biodegradation of these scaffolds is proposed that involves (i) a release of polymer crystallites, (ii) crystallization of the amorphous phase, and (iii) dissolution of the amorphous phase. Incorporation of Fe3O4, Fe3O4–GO, or Fe3O4–rGO accelerated the biodegradation of PHB scaffolds owing to pores on the surface of composite fibers and the enlarged content of polymer amorphous phase in the composite scaffolds. Six-month biodegradation caused a reduction in surface potential (1.5-fold) and in a vertical piezoresponse (3.5-fold) of the Fe3O4–GO scaffold because of a decrease in the PHB β-phase content. In vitro assays in the absence of an MF showed a significantly more pronounced mesenchymal stem cell proliferation on composite magnetic scaffolds compared to the neat scaffold, whereas in an MF (68 mT, 0.67 Hz), cell proliferation was not statistically significantly different when all the studied scaffolds were compared. The PHB/Fe3O4–GO scaffold was implanted into femur bone defects in rats, resulting in successful bone repair after nonperiodic magnetic stimulation (200 mT, 0.04 Hz) owing to a synergetic influence of increased surface roughness, the presence of hydrophilic groups near the surface, and magnetoelectric and magnetomechanical effects of the material Текстовый файл AM_Agreement |
| Taal: | Engels |
| Gepubliceerd in: |
2024
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| Onderwerpen: | |
| Online toegang: | https://doi.org/10.1021/acsami.4c06835 |
| Formaat: | Elektronisch Hoofdstuk |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=678813 |
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| 200 | 1 | |a Osteogenic Potential and Long-Term Enzymatic Biodegradation of PHB-based Scaffolds with Composite Magnetic Nanofillers in a Magnetic Field |f Lada E. Shlapakova, Artyom S. Pryadko, Irina I. Zharkova [et al.] | |
| 203 | |a Текст |c электронный |b визуальный | ||
| 283 | |a online_resource |2 RDAcarrier | ||
| 300 | |a Title screen | ||
| 320 | |a References: 70 tit | ||
| 330 | |a Millions of people worldwide suffer from musculoskeletal damage, thus using the largest proportion of rehabilitation services. The limited self-regenerative capacity of bone and cartilage tissues necessitates the development of functional biomaterials. Magnetoactive materials are a promising solution due to clinical safety and deep tissue penetration of magnetic fields (MFs) without attenuation and tissue heating. Herein, electrospun microfibrous scaffolds were developed based on piezoelectric poly(3-hydroxybutyrate) (PHB) and composite magnetic nanofillers [magnetite with graphene oxide (GO) or reduced GO]. The scaffolds’ morphology, structure, mechanical properties, surface potential, and piezoelectric response were systematically investigated. Furthermore, a complex mechanism of enzymatic biodegradation of these scaffolds is proposed that involves (i) a release of polymer crystallites, (ii) crystallization of the amorphous phase, and (iii) dissolution of the amorphous phase. Incorporation of Fe3O4, Fe3O4–GO, or Fe3O4–rGO accelerated the biodegradation of PHB scaffolds owing to pores on the surface of composite fibers and the enlarged content of polymer amorphous phase in the composite scaffolds. Six-month biodegradation caused a reduction in surface potential (1.5-fold) and in a vertical piezoresponse (3.5-fold) of the Fe3O4–GO scaffold because of a decrease in the PHB β-phase content. In vitro assays in the absence of an MF showed a significantly more pronounced mesenchymal stem cell proliferation on composite magnetic scaffolds compared to the neat scaffold, whereas in an MF (68 mT, 0.67 Hz), cell proliferation was not statistically significantly different when all the studied scaffolds were compared. The PHB/Fe3O4–GO scaffold was implanted into femur bone defects in rats, resulting in successful bone repair after nonperiodic magnetic stimulation (200 mT, 0.04 Hz) owing to a synergetic influence of increased surface roughness, the presence of hydrophilic groups near the surface, and magnetoelectric and magnetomechanical effects of the material | ||
| 336 | |a Текстовый файл | ||
| 371 | 0 | |a AM_Agreement | |
| 461 | 1 | |t ACS Applied Materials and Interfaces |c Washington |n American Chemical Society | |
| 463 | 1 | |t Vol. 16, iss. 42 |v P. 56555–56579 |d 2024 | |
| 610 | 1 | |a electrospun scaffold | |
| 610 | 1 | |a piezoelectricity | |
| 610 | 1 | |a magnetite | |
| 610 | 1 | |a graphene oxide | |
| 610 | 1 | |a enzymatic biodegradation | |
| 610 | 1 | |a bone regeneration | |
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a труды учёных ТПУ | |
| 701 | 1 | |a Shlapakova |b L. E. |c chemical engineer |c Research Engineer of Tomsk Polytechnic University |f 1999- |g Lada Evgenievna |9 88580 | |
| 701 | 1 | |a Pryadko |b A. |c Specialist in the field of nuclear technologies |c Research Engineer of Tomsk Polytechnic University |f 1995- |g Artyom |9 22547 | |
| 701 | 1 | |a Zharkova |b I. I. |g Irina | |
| 701 | 1 | |a Volkov |b A. |g Alexey | |
| 701 | 1 | |a Kozadayeva |b M. |c chemist |c engineer of Tomsk Polytechnic University |f 1998- |g Maria |9 22899 | |
| 701 | 1 | |a Chernozem |b R. V. |c physicist |c Associate Professor of Tomsk Polytechnic University |f 1992- |g Roman Viktorovich |9 19499 | |
| 701 | 1 | |a Mukhortova |b Yu. R. |c Chemical engineer |c Engineer of Tomsk Polytechnic University |f 1976- |g Yulia Ruslanovna |9 22264 | |
| 701 | 1 | |a Chesnokova |b D. |g Dariana | |
| 701 | 1 | |a Zhuikov |b V. A. |g Vsevolod | |
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| 701 | 1 | |a Romanyuk |b K. |g Konstantin | |
| 701 | 1 | |a Kholkin |b A. L. |c physicist |c Director of the International Research Center for PMEM of the Tomsk Polytechnic University, Candidate of Physical and Mathematical Sciences |f 1954- |g Andrei Leonidovich |9 22787 | |
| 701 | 1 | |a Bonartsev |b A. P. |g Anton | |
| 701 | 1 | |a Surmeneva |b M. A. |c specialist in the field of material science |c engineer-researcher of Tomsk Polytechnic University, Associate Scientist |f 1984- |g Maria Alexandrovna |9 15966 | |
| 701 | 1 | |a Surmenev |b R. A. |c physicist |c Associate Professor of Tomsk Polytechnic University, Senior researcher, Candidate of physical and mathematical sciences |f 1982- |g Roman Anatolievich |9 15957 | |
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