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200 1 |a Magnetoactive Composite Conduits Based on Poly(3-hydroxybutyrate) and Magnetite Nanoparticles for Repair of Peripheral Nerve Injury  |f Lada E. Shlapakova, Vladimir V. Botvin, Yulia R. Mukhortova [et al.]  
203 |a Текст  |b визуальный  |c электронный 
283 |a online_resource  |2 RDAcarrier 
300 |a Title screen 
320 |a Peripheral nerve injury poses a threat to the mobility and sensitivity of a nerve, thereby leading to permanent function loss due to the low regenerative capacity of mature neurons. To date, the most widely clinically applied approach to bridging nerve injuries is autologous nerve grafting, which faces challenges such as donor site morbidity, donor shortages, and the necessity of a second surgery. An effective therapeutic strategy is urgently needed worldwide to overcome the current limitations. Herein, a magnetic nerve guidance conduit (NGC) based on biocompatible biodegradable poly(3-hydroxybutyrate) (PHB) and 8 wt % of magnetite nanoparticles modified by citric acid (Fe3O4–CA) was fabricated by electrospinning. The crystalline structure of NGCs was studied by X-ray diffraction, which indicated an enlarged β-phase of PHB in the composite conduit compared to a pure PHB conduit. Tensile tests revealed greater ductility of PHB/Fe3O4–CA: the composite conduit has Young’s modulus of 221 ± 52 MPa and an elongation at break of 28.6 ± 2.9%, comparable to clinical materials. Saturation magnetization (σs) of Fe3O4–CA and PHB/Fe3O4–CA is 61.88 ± 0.29 and 7.44 ± 0.07 emu/g, respectively. The water contact angle of the PHB/Fe3O4–CA conduit is lower as compared to pure PHB, while surface free energy (σ) is significantly higher, which was attributed to higher surface roughness and an amorphous phase as well as possible PHB/Fe3O4–CA interface interactions. In vitro, the conduits supported the proliferation of rat mesenchymal stem cells (rMSCs) and SH-SY5Y cells in a low-frequency magnetic field (0.67 Hz, 68 mT). In vivo, the conduits were used to bridge damaged sciatic nerves in rats; pure PHB and composite PHB/Fe3O4–CA conduits did not cause acute inflammation and performed a barrier function, which promotes nerve regeneration. Thus, these conduits are promising as implants for the regeneration of peripheral nerves. 
336 |a Текстовый файл 
371 |a AM_Agreement 
461 1 |t ACS Applied Bio Materials  |c Washington  |n ACS Publications 
463 1 |t Vol. 2, iss. 7  |v P. 1095-1114  |d 2024 
610 1 |a труды учёных ТПУ 
610 1 |a электронный ресурс 
610 1 |a poly(3-hydroxybutyrate) 
610 1 |a magnetite 
610 1 |a electrospinning 
610 1 |a nerve guidance conduit 
610 1 |a peripheral nerve injury 
701 1 |a Shlapakova  |b L. E.  |c chemical engineer  |c Research Engineer of Tomsk Polytechnic University  |f 1999-  |g Lada Evgenievna  |y Tomsk  |9 88580 
701 1 |a Botvin  |b V. V.  |c chemist  |c Senior Researcher of Tomsk Polytechnic University, Candidate of chemical sciences  |f 1991-  |g Vladimir Viktorovich  |9 22791 
701 1 |a Mukhortova  |b Yu. R.  |c Chemical engineer  |c Engineer of Tomsk Polytechnic University  |f 1976-  |g Yulia Ruslanovna  |9 22264 
701 1 |a Zharkova  |b I. I.  |g Irina Igorevna 
701 1 |a Alipkina  |b S. I.  |g Svetlana 
701 1 |a Zeltzer  |b A.  |g Angelina 
701 1 |a Dudun  |b A. A.  |g Andrey 
701 1 |a Makhina  |b T.  |g Tatjyana 
701 1 |a Bonartseva  |b G. A.  |g Garina Aleksandrovna 
701 1 |a Voinova  |b V. V.  |g Vera 
701 1 |a Vagner  |b D. V.  |g Dmitry Viktorovich 
701 1 |a Pary (Pariy)  |b I. O.  |c physicist  |c engineer of Tomsk Polytechnic University  |f 1995-  |g Igor Olegovich  |9 21904 
701 1 |a Bonartsev   |b A. P.  |g Anton 
701 1 |a Surmenev  |b R. A.  |c physicist  |c Associate Professor of Tomsk Polytechnic University, Senior researcher, Candidate of physical and mathematical sciences  |f 1982-  |g Roman Anatolievich  |9 15957 
701 1 |a Surmeneva  |b M. A.  |c specialist in the field of material science  |c engineer-researcher of Tomsk Polytechnic University, Associate Scientist  |f 1984-  |g Maria Alexandrovna  |9 15966 
712 0 2 |a National Research Tomsk Polytechnic University  |9 27197  |4 570 
801 0 |a RU  |b 63413507  |c 20240703 
856 4 |u https://doi.org/10.1021/acsabm.3c01032  |z https://doi.org/10.1021/acsabm.3c01032 
942 |c CR