A novel impedimetric sensor based on N-acetyl-L-cysteine for the determination of hydroxyl radicals in cell cultures in vitro; Talanta; Vol. 270

A novel impedimetric sensor based on N-acetyl-L-cysteine for the determination of hydroxyl radicals in cell cultures in vitro; Talanta; Vol. 270

Bibliografske podrobnosti
Parent link:	Talanta.— .— Amsterdam: Elsevier Science Publishing Company Inc. Vol. 270.— 2024.— Article number 125600, 8 p.
Drugi avtorji:	Erkovich A. V. Alina Vadimovna, Korotkova E. I. Elena Ivanovna, Dorozhko E. V. Elena Vladimirovna, Plotnikov E. V. Evgeny Vladimirovich, Semin V. O. Viktor Olegovich, Chernova A. P. Anna Pavlovna, Barek J. Jiri, Solomonenko A. N. Anna Nikolaevna, Aseeva N. V. Natalya Valerevna
Izvleček:	Title screen We report a novel impedimetric sensor based on a graphite electrode impregnated with polyethylene and paraffin under vacuum (IGE) modified with electrochemically deposited gold and a self-assembled monolayer of N-acetyl-L-cysteine (NAC/Au/IGE) for selective and sensitive determination of extracellular hydroxyl radicals (OH•) generated by living cells. The application of a sulphur-containing molecule oxidized by OH• predicts the high selectivity of the sensor, and the utilization of the non-faradaic impedance spectroscopy for recording an analytical response makes it possible to achieve superior sensitivity with a detection limit of 0.01 nM and a linear dynamic range of 0.08–8 nM. Meanwhile, NAC/Au/IGE demonstrated a strong potential of detecting OH• generated by biological objects via successful determination of extracellular hydroxyl radicals generated by normal fibroblast cells and prostate carcinoma cells Текстовый файл AM_Agreement
Jezik:	angleščina
Izdano:	2024
Teme:	электронный ресурс труды учёных ТПУ hydroxyl radical impedimetric sensor cell cultures N-Acetyl-L-cysteine oxidative stress
Online dostop:	https://doi.org/10.1016/j.talanta.2023.125600
Format:	Elektronski Book Chapter
KOHA link:	https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=672390

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