A novel impedimetric sensor based on N-acetyl-L-cysteine for the determination of hydroxyl radicals in cell cultures in vitro

A novel impedimetric sensor based on N-acetyl-L-cysteine for the determination of hydroxyl radicals in cell cultures in vitro

Bibliographic Details
Parent link:Talanta.— .— Amsterdam: Elsevier Science Publishing Company Inc.
Vol. 270.— 2024.— Article number 125600, 8 p.
Corporate Author: National Research Tomsk Polytechnic University
Other Authors: Erkovich A. V. Alina Vadimovna, Korotkova E. I. Elena Ivanovna, Dorozhko E. V. Elena Vladimirovna, Plotnikov E. V. Evgeny Vladimirovich, Semin V. O. Viktor Olegovich, Chernova A. P. Anna Pavlovna, Barek J. Jiri, Solomonenko A. N. Anna Nikolaevna, Aseeva N. V. Natalya Valerevna
Summary:Title screen
We report a novel impedimetric sensor based on a graphite electrode impregnated with polyethylene and paraffin under vacuum (IGE) modified with electrochemically deposited gold and a self-assembled monolayer of N-acetyl-L-cysteine (NAC/Au/IGE) for selective and sensitive determination of extracellular hydroxyl radicals (OH•) generated by living cells. The application of a sulphur-containing molecule oxidized by OH• predicts the high selectivity of the sensor, and the utilization of the non-faradaic impedance spectroscopy for recording an analytical response makes it possible to achieve superior sensitivity with a detection limit of 0.01 nM and a linear dynamic range of 0.08–8 nM. Meanwhile, NAC/Au/IGE demonstrated a strong potential of detecting OH• generated by biological objects via successful determination of extracellular hydroxyl radicals generated by normal fibroblast cells and prostate carcinoma cells
AM_Agreement
Language:English
Published: 2024
Subjects:
электронный ресурс
труды учёных ТПУ
hydroxyl radical
impedimetric sensor
cell cultures
N-Acetyl-L-cysteine
oxidative stress
Online Access:https://doi.org/10.1016/j.talanta.2023.125600
Format: Electronic Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=672390

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