A novel impedimetric sensor based on N-acetyl-L-cysteine for the determination of hydroxyl radicals in cell cultures in vitro; Talanta; Vol. 270
| Parent link: | Talanta.— .— Amsterdam: Elsevier Science Publishing Company Inc. Vol. 270.— 2024.— Article number 125600, 8 p. |
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| Andre forfattere: | , , , , , , , , |
| Summary: | Title screen We report a novel impedimetric sensor based on a graphite electrode impregnated with polyethylene and paraffin under vacuum (IGE) modified with electrochemically deposited gold and a self-assembled monolayer of N-acetyl-L-cysteine (NAC/Au/IGE) for selective and sensitive determination of extracellular hydroxyl radicals (OH•) generated by living cells. The application of a sulphur-containing molecule oxidized by OH• predicts the high selectivity of the sensor, and the utilization of the non-faradaic impedance spectroscopy for recording an analytical response makes it possible to achieve superior sensitivity with a detection limit of 0.01 nM and a linear dynamic range of 0.08–8 nM. Meanwhile, NAC/Au/IGE demonstrated a strong potential of detecting OH• generated by biological objects via successful determination of extracellular hydroxyl radicals generated by normal fibroblast cells and prostate carcinoma cells Текстовый файл AM_Agreement |
| Sprog: | engelsk |
| Udgivet: |
2024
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| Fag: | |
| Online adgang: | https://doi.org/10.1016/j.talanta.2023.125600 |
| Format: | xMaterials Electronisk Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=672390 |
MARC
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| 200 | 1 | |a A novel impedimetric sensor based on N-acetyl-L-cysteine for the determination of hydroxyl radicals in cell cultures in vitro |f A. V. Erkovich, E. I. Korotkova, E. V. Dorozhko [et al.] | |
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| 300 | |a Title screen | ||
| 320 | |a References: 27 tit | ||
| 330 | |a We report a novel impedimetric sensor based on a graphite electrode impregnated with polyethylene and paraffin under vacuum (IGE) modified with electrochemically deposited gold and a self-assembled monolayer of N-acetyl-L-cysteine (NAC/Au/IGE) for selective and sensitive determination of extracellular hydroxyl radicals (OH•) generated by living cells. The application of a sulphur-containing molecule oxidized by OH• predicts the high selectivity of the sensor, and the utilization of the non-faradaic impedance spectroscopy for recording an analytical response makes it possible to achieve superior sensitivity with a detection limit of 0.01 nM and a linear dynamic range of 0.08–8 nM. Meanwhile, NAC/Au/IGE demonstrated a strong potential of detecting OH• generated by biological objects via successful determination of extracellular hydroxyl radicals generated by normal fibroblast cells and prostate carcinoma cells | ||
| 336 | |a Текстовый файл | ||
| 371 | 0 | |a AM_Agreement | |
| 461 | 1 | |t Talanta |c Amsterdam |n Elsevier Science Publishing Company Inc. | |
| 463 | 1 | |t Vol. 270 |v Article number 125600, 8 p. |d 2024 | |
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a труды учёных ТПУ | |
| 610 | 1 | |a hydroxyl radical | |
| 610 | 1 | |a impedimetric sensor | |
| 610 | 1 | |a cell cultures | |
| 610 | 1 | |a N-Acetyl-L-cysteine | |
| 610 | 1 | |a oxidative stress | |
| 701 | 1 | |a Erkovich |b A. V. |c chemical technologist |c engineer of Tomsk Polytechnic University |f 1997- |g Alina Vadimovna |9 23015 | |
| 701 | 1 | |a Korotkova |b E. I. |c chemist |c Professor of Tomsk Polytechnic University, Doctor of chemical science |f 1965- |g Elena Ivanovna |9 15456 | |
| 701 | 1 | |a Dorozhko |b E. V. |c chemist |c Engineer of Tomsk Polytechnic University |f 1979- |g Elena Vladimirovna |9 16175 | |
| 701 | 1 | |a Plotnikov |b E. V. |c chemist |c Associate Professor of Tomsk Polytechnic University, Candidate of Chemical Sciences |f 1983- |g Evgeny Vladimirovich |9 16417 | |
| 701 | 1 | |a Semin |b V. O. |g Viktor Olegovich | |
| 701 | 1 | |a Chernova |b A. P. |c chemist |c Associate Professor of Tomsk Polytechnic University, Candidate of chemical sciences |f 1984- |g Anna Pavlovna |9 22278 | |
| 701 | 1 | |a Barek |b J. |c Czech chemist |c Professor of Tomsk Polytechnic University, candidate of chemical Sciences |f 1949- |g Jiri |9 21069 | |
| 701 | 1 | |a Solomonenko |b A. N. |c Chemical engineer |c Engineer of Tomsk Polytechnic University |f 1995- |g Anna Nikolaevna |9 22606 | |
| 701 | 1 | |a Aseeva |b N. V. |c Chemical engineer |c Engineer of Tomsk Polytechnic University |f 1995- |g Natalya Valerevna |9 22125 | |
| 801 | 0 | |a RU |b 63413507 |c 20240506 |g RCR | |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.talanta.2023.125600 |z https://doi.org/10.1016/j.talanta.2023.125600 |
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