Peptide ligands on the PEGylated nanoparticle surface and human serum composition are key factors for the interaction between immune cells and nanoparticles; Colloids and Surfaces B: Biointerfaces; Vol. 221

Peptide ligands on the PEGylated nanoparticle surface and human serum composition are key factors for the interaction between immune cells and nanoparticles; Colloids and Surfaces B: Biointerfaces; Vol. 221

Bibliographic Details
Parent link:Colloids and Surfaces B: Biointerfaces
Vol. 221.— 2023.— [112981, 13 p.]
Corporate Author: Национальный исследовательский Томский политехнический университет Исследовательская школа химических и биомедицинских технологий
Other Authors: Pershina A. G. Aleksandra Gennadievna, Demin A. M., Perekucha N. A. Nataljya Andreevna, Brikunova O. Ya. Olga Yaroslavovna, Efimova L. V. Lina Viktorovna, Nevskaya K. V., Vakhrushev A. V. Aleksandr, Zgoda V. G. Viktor, Uymin M. A. Mikhail Aleksandrovich, Minin A. S. Artyom, Malkeeva D. Dina, Kiseleva E. Elena, Zima A. P. Anastasiya Pavlovna, Krasnov V. P. Viktor, Ogorodova L. M. Lyudmila Mikhaylovna
Summary:Title screen
The architecture of a nanoparticles’ surface formed due to a modification with a ligand and protein corona formation in biofluids is critical for interactions with cells in vivo. Here we studied interactions of immune cells with magnetic nanoparticles (MNPs) covalently modified with polyethylene glycol (PEG) and their counterparts conjugated with peptides: a pH (low) insertion peptide (pHLIP) and cycloRGD as a targeting ligand in human serum. The conjugation of MNPs-PEG with pHLIP, but not with cycloRGD, enhanced the association of these particles with mononuclear phagocytic cells in vitro and in vivo. We did not find a clear difference in protein corona composition between the pHLIP-modified and parental PEGylated nanoparticles. Analysis of the effect of autologous human serum on MNP uptake by monocytes showed that the efficiency of endocytosis varies among healthy donors and depends on intrinsic properties of serum. Nevertheless, using classic blood, coagulation, biochemical tests, and anti-PEG IgG serum level, we failed to identify the cause of the observed interdonor variation. These individual differences should be taken into consideration during testing of nanotherapeutics.
Режим доступа: по договору с организацией-держателем ресурса
Language:English
Published: 2023
Subjects:
электронный ресурс
труды учёных ТПУ
immune cells
Online Access:https://doi.org/10.1016/j.colsurfb.2022.112981
Format: Electronic Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=669441

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