Boosting transfection efficiency: A systematic study using layer-by-layer based gene delivery platform; Materials Science and Engineering: C; Vol. 126
| Parent link: | Materials Science and Engineering: C Vol. 126.— 2021.— [112161, 11 p.] |
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| Autor corporatiu: | |
| Altres autors: | , , , , , , , , |
| Sumari: | Title screen Nowadays, the nanoparticle-based delivery approach is becoming more and more attractive in gene therapy due to its low toxicity and immunogenicity, sufficient packaging capacity, targeting, and straightforward, low-cost, large-scale good manufacturing practice (GMP) production. A number of research works focusing on multilayer structures have explored different factors and parameters that can affect the delivery efficiency of pDNA. However, there are no systematic studies on the performance of these structures for enhanced gene delivery regarding the gene loading methods, the use of additional organic components and cell/particle incubation conditions. Here, we conducted a detailed analysis of different parameters such as (i) strategy for loading pDNA into carriers, (ii) incorporating both pDNA and organic additives within one carrier and (iii) variation of cell/particle incubation conditions, to evaluate their influence on the efficiency of pDNA delivery with multilayer structures consisting of inorganic cores and polymer layers. Our results reveal that an appropriate combination of all these parameters leads to the development of optimized protocols for high transfection efficiency, compared to the non-optimized process (> 70% vs. < 7%), and shows a good safety profile. In conclusion, we provide the proof-of-principle that these multilayer structures with the developed parameters are a promising non-viral platform for an efficient delivery of nucleic acids. Режим доступа: по договору с организацией-держателем ресурса |
| Idioma: | anglès |
| Publicat: |
2021
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| Matèries: | |
| Accés en línia: | https://doi.org/10.1016/j.msec.2021.112161 |
| Format: | Electrònic Capítol de llibre |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=667957 |
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| 200 | 1 | |a Boosting transfection efficiency: A systematic study using layer-by-layer based gene delivery platform |f Ya. V. Tarakanchikova, D. S. Linnik, T. V. Mashel [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: 47 tit.] | ||
| 330 | |a Nowadays, the nanoparticle-based delivery approach is becoming more and more attractive in gene therapy due to its low toxicity and immunogenicity, sufficient packaging capacity, targeting, and straightforward, low-cost, large-scale good manufacturing practice (GMP) production. A number of research works focusing on multilayer structures have explored different factors and parameters that can affect the delivery efficiency of pDNA. However, there are no systematic studies on the performance of these structures for enhanced gene delivery regarding the gene loading methods, the use of additional organic components and cell/particle incubation conditions. Here, we conducted a detailed analysis of different parameters such as (i) strategy for loading pDNA into carriers, (ii) incorporating both pDNA and organic additives within one carrier and (iii) variation of cell/particle incubation conditions, to evaluate their influence on the efficiency of pDNA delivery with multilayer structures consisting of inorganic cores and polymer layers. Our results reveal that an appropriate combination of all these parameters leads to the development of optimized protocols for high transfection efficiency, compared to the non-optimized process (> 70% vs. < 7%), and shows a good safety profile. In conclusion, we provide the proof-of-principle that these multilayer structures with the developed parameters are a promising non-viral platform for an efficient delivery of nucleic acids. | ||
| 333 | |a Режим доступа: по договору с организацией-держателем ресурса | ||
| 338 | |b Российский научный фонд |d 20-45-01012 | ||
| 338 | |b Российский фонд фундаментальных исследований |d 19-29-04025 | ||
| 461 | |t Materials Science and Engineering: C | ||
| 463 | |t Vol. 126 |v [112161, 11 p.] |d 2021 | ||
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| 610 | 1 | |a gene delivery | |
| 610 | 1 | |a calcium carbonate | |
| 610 | 1 | |a organic additives | |
| 610 | 1 | |a DNase inhibitor | |
| 610 | 1 | |a cell incubation protocol | |
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| 610 | 1 | |a ингибиторы | |
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| 701 | 1 | |a Tarakanchikova |b Ya. V. |g Yana Vladimirovna | |
| 701 | 1 | |a Linnik |b D. S. |g Dmitrii Sergeevich | |
| 701 | 1 | |a Mashel |b T. V. |g Tatjyana Vyacheslavovna | |
| 701 | 1 | |a Muslimov |b A. R. |g Albert | |
| 701 | 1 | |a Pavlov |b S. I. |g Sergey Igorevich | |
| 701 | 1 | |a Lepik |b K. V. |g Kirill Viktorovich | |
| 701 | 1 | |a Zyuzin |b M. V. |g Mikhail | |
| 701 | 1 | |a Sukhorukov |b G. B. |g Gleb Borisovich | |
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