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| LEADER |
00000naa0a2200000 4500 |
| 001 |
667813 |
| 005 |
20250403164647.0 |
| 035 |
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|a (RuTPU)RU\TPU\network\39024
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|a 667813
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| 100 |
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|a 20220422d2022 k||y0rusy50 ba
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0 |
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|a eng
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| 135 |
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|a drcn ---uucaa
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| 181 |
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0 |
|a i
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| 182 |
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0 |
|a b
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| 200 |
1 |
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|a Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells
|f D. Facchetti, U. Hempel, L. Martocq [et al.]
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| 203 |
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|a Text
|c electronic
|
| 320 |
|
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|a [References: 30 tit.]
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| 330 |
|
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|a Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion. The success of Ti6Al4V implants depends on bone formation on the implant surface. Hence, implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells are desirable. One coating strategy is by adsorption of biomacromolecules. In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells. WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC). Indeed, WPI fibrils were resistant to sterilization and were stable during storage. This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity. Finally, the coating increased the hydrophilicity of the material. The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments. Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
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| 461 |
|
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|t International Journal of Molecular Sciences
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| 463 |
|
|
|t Vol. 23, iss. 10
|v [139, 12 p. ]
|d 2022
|
| 610 |
1 |
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|a труды учёных ТПУ
|
| 610 |
1 |
|
|a электронный ресурс
|
| 610 |
1 |
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|a WPI fibrils
|
| 610 |
1 |
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|a Ti6Al4V
|
| 610 |
1 |
|
|a additive manufacturing
|
| 610 |
1 |
|
|a osseointegration
|
| 610 |
1 |
|
|a heparin; tinzaparin
|
| 610 |
1 |
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|a osteoblast differentiation
|
| 610 |
1 |
|
|a coating; enriched
|
| 701 |
|
1 |
|a Facchetti
|b D.
|g Davide
|
| 701 |
|
1 |
|a Hempel
|b U.
|g Ute
|
| 701 |
|
1 |
|a Martocq
|b L.
|g Laurine
|
| 701 |
|
1 |
|a Smith
|b A. M.
|g Alan
|
| 701 |
|
1 |
|a Koptyug
|b A. V.
|g Andrey Valentinovich
|
| 701 |
|
1 |
|a Surmenev
|b R. A.
|c physicist
|c Associate Professor of Tomsk Polytechnic University, Senior researcher, Candidate of physical and mathematical sciences
|f 1982-
|g Roman Anatolievich
|3 (RuTPU)RU\TPU\pers\31885
|9 15957
|
| 701 |
|
1 |
|a Surmeneva
|b M. A.
|c specialist in the field of material science
|c engineer-researcher of Tomsk Polytechnic University, Associate Scientist
|f 1984-
|g Maria Alexandrovna
|3 (RuTPU)RU\TPU\pers\31894
|9 15966
|
| 701 |
|
1 |
|a Douglas
|b T. E. L.
|g Timothy
|
| 712 |
0 |
2 |
|a Национальный исследовательский Томский политехнический университет
|b Исследовательская школа химических и биомедицинских технологий
|c (2017- )
|3 (RuTPU)RU\TPU\col\23537
|
| 801 |
|
0 |
|a RU
|b 63413507
|c 20220422
|g RCR
|
| 856 |
4 |
|
|u https://doi.org/10.3390/ijms23010139
|
| 942 |
|
|
|c CF
|
