Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood; Sensors; Vol. 21, iss. 23

Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood; Sensors; Vol. 21, iss. 23

Bibliographic Details
Parent link:Sensors
Vol. 21, iss. 23.— 2021.— [7851, 20 p.]
Corporate Author: Национальный исследовательский Томский политехнический университет Инженерная школа новых производственных технологий Отделение материаловедения
Other Authors: Shabalina A. Anastasiia, Sharko D. Darya, Glazyrin Yu. Yury, Bolshevich E. Elena, Dubinina O. V. Oksana Valerievna, Kim A. Anastasiia, Veprintsev D. Dmitry, Lapin I. Ivan, Zamay G. Galina, Krat A. Alexey, Zamay S. Sergey, Svetlichnyi V. Valery, Kichkailo A. Anna, Berezovski M. Maxim
Summary:Title screen
We describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability. A new blocking agent, a thiolated oligonucleotide with an unrelated sequence, was applied to fill the aptamer layer’s defects. Electrochemical aptasensor signal processing was enhanced using deep learning and computer simulation of the experimental data array. It was found that the combinations (coupled and tripled) of cyclic voltammogram features allowed for distinguishing between the samples from lung cancer patients and healthy candidates with a mean accuracy of 0.73. The capacitive component from the non-Faradic electrochemical impedance spectroscopy data indicated the tumor marker’s presence in a sample. These findings allowed for the creation of highly informative aptasensors for early lung cancer diagnostics.
Language:English
Published: 2021
Subjects:
электронный ресурс
труды учёных ТПУ
electrochemical aptasensor
biosensing layer
useful signal
lung cancer
human blood
биосенсоры
Online Access:https://doi.org/10.3390/s21237851
Format: Electronic Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=667257

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