Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli; Protein Expression and Purification; Vol. 143

Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli; Protein Expression and Purification; Vol. 143

Λεπτομέρειες βιβλιογραφικής εγγραφής
Parent link:Protein Expression and Purification
Vol. 143.— 2018.— [P. 38-44]
Συγγραφή απο Οργανισμό/Αρχή: Национальный исследовательский Томский политехнический университет Исследовательская школа химических и биомедицинских технологий
Άλλοι συγγραφείς: Ashcheulova D. O., Efimova L. V. Lina Viktorovna, Lushchyk A. Y., Yantsevich A. V., Baykov A. N. Aleksandr Nikolaevich, Pershina A. G. Aleksandra Gennadievna
Περίληψη:Title screen
Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand.
Γλώσσα:Αγγλικά
Έκδοση: 2018
Θέματα:
труды учёных ТПУ
электронный ресурс
antimicrobial peptides
ubiquicidine
ketosteroid isomerase
size exclusion chromatography
Inclusion bodies
компоненты
антимикробные пептиды
диагностические системы
эксклюзионная хроматография
Διαθέσιμο Online:https://doi.org/10.1016/j.pep.2017.10.011
Μορφή: Ηλεκτρονική πηγή Κεφάλαιο βιβλίου
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=667254

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