Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli; Protein Expression and Purification; Vol. 143
| Parent link: | Protein Expression and Purification Vol. 143.— 2018.— [P. 38-44] |
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| Korporativna značnica: | |
| Drugi avtorji: | , , , , , |
| Izvleček: | Title screen Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand. |
| Jezik: | angleščina |
| Izdano: |
2018
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| Teme: | |
| Online dostop: | https://doi.org/10.1016/j.pep.2017.10.011 |
| Format: | Elektronski Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=667254 |
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| 200 | 1 | |a Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli |f D. O. Ashcheulova, L. V. Efimova, A. Y. Lushchyk [et. al] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: 22 tit.] | ||
| 330 | |a Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand. | ||
| 461 | |t Protein Expression and Purification | ||
| 463 | |t Vol. 143 |v [P. 38-44] |d 2018 | ||
| 610 | 1 | |a труды учёных ТПУ | |
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a antimicrobial peptides | |
| 610 | 1 | |a ubiquicidine | |
| 610 | 1 | |a ketosteroid isomerase | |
| 610 | 1 | |a size exclusion chromatography | |
| 610 | 1 | |a Inclusion bodies | |
| 610 | 1 | |a компоненты | |
| 610 | 1 | |a антимикробные пептиды | |
| 610 | 1 | |a диагностические системы | |
| 610 | 1 | |a эксклюзионная хроматография | |
| 701 | 1 | |a Ashcheulova |b D. O. | |
| 701 | 1 | |a Efimova |b L. V. |g Lina Viktorovna | |
| 701 | 1 | |a Lushchyk |b A. Y. | |
| 701 | 1 | |a Yantsevich |b A. V. | |
| 701 | 1 | |a Baykov |b A. N. |g Aleksandr Nikolaevich | |
| 701 | 1 | |a Pershina |b A. G. |c biologist |c Associate Professor of Tomsk Polytechnic University, Candidate of biological sciences |f 1981- |g Aleksandra Gennadievna |3 (RuTPU)RU\TPU\pers\32466 |9 16414 | |
| 712 | 0 | 2 | |a Национальный исследовательский Томский политехнический университет |b Исследовательская школа химических и биомедицинских технологий |c (2017- ) |3 (RuTPU)RU\TPU\col\23537 |
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| 856 | 4 | |u https://doi.org/10.1016/j.pep.2017.10.011 | |
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