|
|
|
|
| LEADER |
00000naa0a2200000 4500 |
| 001 |
664949 |
| 005 |
20251117110523.0 |
| 035 |
|
|
|a (RuTPU)RU\TPU\network\36134
|
| 035 |
|
|
|a RU\TPU\network\35057
|
| 090 |
|
|
|a 664949
|
| 100 |
|
|
|a 20210602d2021 k||y0rusy50 ba
|
| 101 |
0 |
|
|a eng
|
| 135 |
|
|
|a drcn ---uucaa
|
| 181 |
|
0 |
|a i
|
| 182 |
|
0 |
|a b
|
| 200 |
1 |
|
|a Preclinical Evaluation of 99mTc-ZHER2:41071, a Second-Generation Affibody-Based HER2-Visualizing Imaging Probe with a Low Renal Uptake
|f M. Oroujeni, S. S. Rinne, A. G. Vorobjeva (Vorobyeva) [et al.]
|
| 203 |
|
|
|a Text
|c electronic
|
| 320 |
|
|
|a [References: 50 tit.]
|
| 330 |
|
|
|a Radionuclide imaging of HER2 expression in tumours may enable stratification of patients with breast, ovarian, and gastroesophageal cancers for HER2-targeting therapies. A first-generation HER2-binding affibody molecule [99mTc]Tc-ZHER2:V2 demonstrated favorable imaging properties in preclinical studies. Thereafter, the affibody scaffold has been extensively modified, which increased its melting point, improved storage stability, and increased hydrophilicity of the surface. In this study, a second-generation affibody molecule (designated ZHER2:41071) with a new improved scaffold has been prepared and characterized. HER2-binding, biodistribution, and tumour-targeting properties of [99mTc]Tc-labelled ZHER2:41071 were investigated. These properties were compared with properties of the first-generation affibody molecules, [99mTc]Tc-ZHER2:V2 and [99mTc]Tc-ZHER2:2395. [99mTc]Tc-ZHER2:41071 bound specifically to HER2 expressing cells with an affinity of 58 ± 2 pM. The renal uptake for [99mTc]Tc-ZHER2:41071 and [99mTc]Tc-ZHER2:V2 was 25–30 fold lower when compared with [99mTc]Tc-ZHER2:2395. The uptake in tumour and kidney for [99mTc]Tc-ZHER2:41071 and [99mTc]Tc-ZHER2:V2 in SKOV-3 xenografts was similar. In conclusion, an extensive re-engineering of the scaffold did not compromise imaging properties of the affibody molecule labelled with 99mTc using a GGGC chelator. The new probe, [99mTc]Tc-ZHER2:41071 provided the best tumour-to-blood ratio compared to HER2-imaging probes for single photon emission computed tomography (SPECT) described in the literature so far. [99mTc]Tc-ZHER2:41071 is a promising candidate for further clinical translation studies.
|
| 461 |
|
|
|t International Journal of Molecular Sciences
|
| 463 |
|
|
|t Vol. 22, iss. 5
|v [2770, 20 p. ]
|d 2021
|
| 610 |
1 |
|
|a труды учёных ТПУ
|
| 610 |
1 |
|
|a электронный ресурс
|
| 610 |
1 |
|
|a HER2
|
| 610 |
1 |
|
|a radionuclide molecular imaging
|
| 610 |
1 |
|
|a affibody molecule
|
| 610 |
1 |
|
|a technetium-99m
|
| 610 |
1 |
|
|a second-generation scaffold
|
| 610 |
1 |
|
|a визуализация
|
| 610 |
1 |
|
|a технеций-99м
|
| 701 |
|
1 |
|a Oroujeni
|b M.
|g Maryam
|
| 701 |
|
1 |
|a Rinne
|b S. S.
|g Sara
|
| 701 |
|
1 |
|a Vorobjeva (Vorobyeva)
|b A. G.
|c specialist in the field of medical technology
|c Senior Researcher, Oncoteranostika Research Center, Tomsk Polytechnic University, Ph.D
|f 1990-
|g Anzhelika Grigorjevna
|3 (RuTPU)RU\TPU\pers\46556
|9 22214
|
| 701 |
|
1 |
|a Loftenius
|b A.
|g Annika
|
| 701 |
|
1 |
|a Feldwisch
|b J.
|g Joachim
|
| 701 |
|
1 |
|a Jonasson
|b P.
|g Per
|
| 701 |
|
1 |
|a Chernov
|b V. I.
|c specialist in the field of medical technology
|c lead engineer of Tomsk Polytechnic University, doctor of medical sciences
|f 1962-
|g Vladimir Ivanovich
|3 (RuTPU)RU\TPU\pers\34191
|9 17725
|
| 701 |
|
1 |
|a Orlova
|b A. M.
|c specialist in the field of medical technology
|c Senior Researcher, Oncoteranostika Research Center, Tomsk Polytechnic University, Ph.D
|f 1960-
|g Anna Markovna
|3 (RuTPU)RU\TPU\pers\46554
|9 22212
|
| 701 |
|
1 |
|a Frejd
|b F. Y.
|g Fredrik
|
| 701 |
|
1 |
|a Tolmachev
|b V. M.
|c specialist in the field of medical technology
|c Director of the Research Center "Oncoteranostika", Tomsk Polytechnic University, Ph.D
|f 1961-
|g Vladimir Maksimilianovich
|3 (RuTPU)RU\TPU\pers\46552
|9 22210
|
| 712 |
0 |
2 |
|a Национальный исследовательский Томский политехнический университет
|b Исследовательская школа химических и биомедицинских технологий
|b Научно-исследовательский центр "Онкотераностика"
|3 (RuTPU)RU\TPU\col\27561
|
| 801 |
|
2 |
|a RU
|b 63413507
|c 20211209
|g RCR
|
| 856 |
4 |
|
|u https://doi.org/10.3390/ijms22052770
|
| 942 |
|
|
|c CF
|
