Dual Targeting of Cancer Cells with DARPin-Based Toxins for Overcoming Tumor Escape; Cancers; Vol. 12, iss. 10
| Parent link: | Cancers Vol. 12, iss. 10.— 2020.— [3014, 15 p.] |
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| Institution som forfatter: | |
| Andre forfattere: | , , , , , , , , , |
| Summary: | Title screen We report here a combined anti-cancer therapy directed toward HER2 and EpCAM, common tumor-associated antigens of breast cancer cells. The combined therapeutic effect is achieved owing to two highly toxic proteins—a low immunogenic variant of Pseudomonas aeruginosa exotoxin A and ribonuclease Barnase from Bacillus amyloliquefaciens. The delivery of toxins to cancer cells was carried out by targeting designed ankyrin repeat proteins (DARPins). We have shown that both target agents efficiently accumulate in the tumor. Simultaneous treatment of breast carcinoma-bearing mice with anti-EpCAM fusion toxin based on LoPE and HER2-specific liposomes loaded with Barnase leads to concurrent elimination of primary tumor and metastases. Monotherapy with anti-HER2- or anti-EpCAM-toxins did not produce a comparable effect on metastases. The proposed approach can be considered as a promising strategy for significant improvement of cancer therapy. |
| Sprog: | engelsk |
| Udgivet: |
2020
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| Fag: | |
| Online adgang: | https://doi.org/10.3390/cancers12103014 |
| Format: | Electronisk Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=664919 |
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| 200 | 1 | |a Dual Targeting of Cancer Cells with DARPin-Based Toxins for Overcoming Tumor Escape |f E. I. Shramova, G. M. Proshkina, V. Shipunova и [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: 52 tit.] | ||
| 330 | |a We report here a combined anti-cancer therapy directed toward HER2 and EpCAM, common tumor-associated antigens of breast cancer cells. The combined therapeutic effect is achieved owing to two highly toxic proteins—a low immunogenic variant of Pseudomonas aeruginosa exotoxin A and ribonuclease Barnase from Bacillus amyloliquefaciens. The delivery of toxins to cancer cells was carried out by targeting designed ankyrin repeat proteins (DARPins). We have shown that both target agents efficiently accumulate in the tumor. Simultaneous treatment of breast carcinoma-bearing mice with anti-EpCAM fusion toxin based on LoPE and HER2-specific liposomes loaded with Barnase leads to concurrent elimination of primary tumor and metastases. Monotherapy with anti-HER2- or anti-EpCAM-toxins did not produce a comparable effect on metastases. The proposed approach can be considered as a promising strategy for significant improvement of cancer therapy. | ||
| 461 | |t Cancers | ||
| 463 | |t Vol. 12, iss. 10 |v [3014, 15 p.] |d 2020 | ||
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a труды учёных ТПУ | |
| 610 | 1 | |a barnase | |
| 610 | 1 | |a liposomes | |
| 610 | 1 | |a HER2 | |
| 610 | 1 | |a EpCAM | |
| 610 | 1 | |a cancer therapy | |
| 610 | 1 | |a липосомы | |
| 610 | 1 | |a противораковая активность | |
| 610 | 1 | |a терапия | |
| 701 | 1 | |a Shramova |b E. I. |g Elena Ivanovna | |
| 701 | 1 | |a Proshkina |b G. M. |g Galina Mikhaylovna | |
| 701 | 1 | |a Shipunova |b V. |g Viktoriya | |
| 701 | 1 | |a Ryabova |b A. |g Anastasiya | |
| 701 | 1 | |a Kamyshinsky |b R. |g Roman | |
| 701 | 1 | |a Konevega |b A. |g Andrey | |
| 701 | 1 | |a Shulga (Schulga) |b A. A. |c biologist |c Researcher, Tomsk Polytechnic University, Candidate of Biological Sciences |f 1960- |g Aleksey Anatolievich |9 22432 | |
| 701 | 1 | |a Konovalova |b E. V. |c specialist in the field of chemical technology and biotechnology |c engineer at Tomsk Polytechnic University |f 1985- |g Elena Valerjevna |9 88562 | |
| 701 | 1 | |a Telegin |b G. |g Georgy | |
| 701 | 1 | |a Deev |b S. M. |c biologist |c Leading Researcher, Tomsk Polytechnic University, Doctor of Biological Sciences |f 1951- |g Sergey Mikhaylovich |3 (RuTPU)RU\TPU\pers\39299 | |
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| 856 | 4 | |u https://doi.org/10.3390/cancers12103014 | |
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