Universal platform for sers detection of "normal" and "tumor" cells cultivation media; NANOCON 2018

Universal platform for sers detection of "normal" and "tumor" cells cultivation media; NANOCON 2018

Bibliographic Details
Parent link:NANOCON 2018.— 2019.— [P. 354-359]
Corporate Author: Национальный исследовательский Томский политехнический университет Исследовательская школа химических и биомедицинских технологий
Other Authors: Erzina M. R. Mariya Rashidovna, Trelin A. Andrey, Guselnikova O. A. Olga Andreevna, Kalachyova E. Evgeniya, Perminova A. Anastasiya, Postnikov P. S. Pavel Sergeevich, Svorcik V. Vaclav, Lyutakov O. Oleksy
Summary:Title screen
Surface-enhanced Raman scattering (SERS) has become an attractive analytical tool for recognition of various biomolecules and bio-objects. In this work, we created a universal platform, based on surface modified gold multibranched nanoparticles (AuMs), plasmon coupling between surface plasmon polariton waves (SPP) and localized surface plasmon (LSP), and utilization of advanced statistical methods for analysis and estimation of SERS spectra, measured on the „tumor“ cells cultivation media. At the first stage, we used the AuMs which have an extremely high number of plasmonic hot spots per single nanoparticle and provide excellent SERS signal intensification. The surface of AuMs was decorated with charged organic functional moieties to entrap various (bio) molecules from targeted solution. Chemical and morphological structures of the prepared nanoparticles were examined with different techniques such as SEM-EDX and Raman spectroscopy. The decorated AuMs were placed in the cultivation media of melanoma and fibroblast lines in vitro. After interaction with cultivation media the AuMs were drop-deposited on the periodical gold grating, to achieve SPP-LSP coupling and even more enhance the SERS signal from entrapped (bio)molecules. Raman spectra were collected from several kinds of melanoma cells lines and evaluated using the developed mathematical route.
Language:English
Published: 2019
Subjects:
труды учёных ТПУ
электронный ресурс
gold nanoparticles
SERS detection
cultivation media
arenediazonium tosylates
наночастицы
золото
питательные среды
Online Access:https://www.confer.cz/nanocon/2018/204-universal-platform-for-sers-detection-of-normal-and-tumor-cells-cultivation-media
Format: Electronic Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=664872

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