Изучение кинетики высвобождения доксорубицина под воздействием ультразвукового излучения при различных значениях рН из носителя на основе микрочастиц Fe(0)
| Parent link: | Вопросы биологической, медицинской и фармацевтической химии Т. 22, № 5.— 2019.— [С. 15-19] |
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| Other Authors: | , , , , , , , |
| Summary: | Заглавие с экрана Исследовано высвобождение доксорубицина из носителя на основе микрочастиц ноль-валентного железа (Fe(0)) при различных значениях рН. Проведено изучение влияния ультразвукового излучения на скорость высвобождения доксорубицина. Сделан вывод о возможности рассмотренной системы доставки лекарственных средств на основе микрочастиц Fe(0) в качестве средства для контролируемого высвобождения доксорубицина. Aim. To obtain new drug carrier for doxorubicin based on modified Fe(0) microparticles and evaluate its release kinetics under influence of different values of pH and ultrasound irradiation. Material and methods. Size and zeta-potential of microparticles were determined on Zetasizer Nano ZS. Surface modification (covalent binding of residues of benzoic acid) was confirmed by FTIR spectroscopy. Encapsulation efficiency (EE) and loading capacity (LC) of doxorubicin (DOX) was determined by UV spectroscopy (480 nm). Release studies were carried out in Stuart SI 500 incubator at a constant temperature (37 °C), stirring rate (100 rpm) and different pH values (3.3; 5.5; 7.4). For investigation of influence of ultrasound (US) irradiation on the release kinetics ultrasound field with frequency and power 75 kHz and 2 W/cm2 respectively was used. Ultrasonic bath Elmasonic S10H was used as a source of ultrasound irradiation. Results and discussions. Size and zeta-potential of Fe-CS-DOX conjugate were 4.43 and -9.07 respectively. Loading capacity of doxorubicin was 0.54 mg/mg. Percentage of released drug with and without US irradiation were 96 and 18% respectively (in 12 hours after starting of the experiment). Conclusion. In this study, the release of doxorubicin from drug carrier, based on Fe (0) microparticles at different pH values was investigated and the influence of ultrasound irradiation on the release kinetics was confirmed. In 12 hours after starting of the release, the amount of released drug was increased more than 4 times. So, the obtained conjugate Fe-CS-DOX leaves great promise for its further use as a drug carrier. Режим доступа: по договору с организацией-держателем ресурса |
| Language: | Russian |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://www.elibrary.ru/item.asp?id=37597573 https://doi.org/10.29296/25877313-2019-05-03 |
| Format: | Electronic Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=664614 |
| Summary: | Заглавие с экрана Исследовано высвобождение доксорубицина из носителя на основе микрочастиц ноль-валентного железа (Fe(0)) при различных значениях рН. Проведено изучение влияния ультразвукового излучения на скорость высвобождения доксорубицина. Сделан вывод о возможности рассмотренной системы доставки лекарственных средств на основе микрочастиц Fe(0) в качестве средства для контролируемого высвобождения доксорубицина. Aim. To obtain new drug carrier for doxorubicin based on modified Fe(0) microparticles and evaluate its release kinetics under influence of different values of pH and ultrasound irradiation. Material and methods. Size and zeta-potential of microparticles were determined on Zetasizer Nano ZS. Surface modification (covalent binding of residues of benzoic acid) was confirmed by FTIR spectroscopy. Encapsulation efficiency (EE) and loading capacity (LC) of doxorubicin (DOX) was determined by UV spectroscopy (480 nm). Release studies were carried out in Stuart SI 500 incubator at a constant temperature (37 °C), stirring rate (100 rpm) and different pH values (3.3; 5.5; 7.4). For investigation of influence of ultrasound (US) irradiation on the release kinetics ultrasound field with frequency and power 75 kHz and 2 W/cm2 respectively was used. Ultrasonic bath Elmasonic S10H was used as a source of ultrasound irradiation. Results and discussions. Size and zeta-potential of Fe-CS-DOX conjugate were 4.43 and -9.07 respectively. Loading capacity of doxorubicin was 0.54 mg/mg. Percentage of released drug with and without US irradiation were 96 and 18% respectively (in 12 hours after starting of the experiment). Conclusion. In this study, the release of doxorubicin from drug carrier, based on Fe (0) microparticles at different pH values was investigated and the influence of ultrasound irradiation on the release kinetics was confirmed. In 12 hours after starting of the release, the amount of released drug was increased more than 4 times. So, the obtained conjugate Fe-CS-DOX leaves great promise for its further use as a drug carrier. Режим доступа: по договору с организацией-держателем ресурса |
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| DOI: | 10.29296/25877313-2019-05-03 |