Behavioral Changes of Multipotent Mesenchymal Stromal Cells in Contact with Synthetic Calcium Phosphates in vitro
| Parent link: | Cell and Tissue Biology Vol. 12, iss. 2.— 2018.— [P. 112–119] |
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| Другие авторы: | , , , , , , , , , , , , |
| Примечания: | Title screen Migration, proliferation, and osteogenic differentiation of human adipose-derived (AD) multipotent mesenchymal stromal cells (MMSCs) during in vitro modeling of indirect contact with calcium phosphate (CP) or nanoparticles of synthetic hydroxyapatite (HA) have been studied. The results were registered with electrode (real-time cell analysis, RTCA) or visual (Cell-IQ) systems of long-term observation of cell cultures. Bulk specimens were use in a Cell-IQ® v2 MLF device as pure titanium substrates (10 Ч 10 Ч 1 mm3) covered by a CP relief (roughness index Ra = 2.4–4.4 µm) bilateral coating that was prepared by the micr-arc method from an aqueous solution of orthophosphoric acid (20 wt %), calcium carbonate (9 wt %), and synthetic HA (6 wt %). HA crystallites (1 mg/mL) were fabricated by mechanochemical synthesis and served as an irritant in RTCA investigation. The Cell-IQ system identified a 3.5- to 10-fold decrease in cell number at the interface with CP coatings with differing roughness during 14-day cell culturing. After 21 days, it was accompanied by a weak reduction of MMSC antigen expression (CD73, CD90, and CD105) as opposed to an increase in MMSC osteogenic differentiation and intercellular-matrix mineralization. In turn, HA nanodispersion reduced the speed of MMSC migration by 1.5 times (P < 0.001) during 25-h RTCA recording, which simulated cell invasion through the microporous membrane (8-µm diameter). Inhibition of migration and cell division with increased osteogenic differentiation of MMSCs has been suggested to be a possible effect of biodegradation products of synthetic CP materials. Режим доступа: по договору с организацией-держателем ресурса |
| Язык: | английский |
| Опубликовано: |
2018
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| Предметы: | |
| Online-ссылка: | https://doi.org/10.1134/S1990519X18020062 |
| Формат: | Электронный ресурс Статья |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=663934 |
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| 200 | 1 | |a Behavioral Changes of Multipotent Mesenchymal Stromal Cells in Contact with Synthetic Calcium Phosphates in vitro |f L. S. Livinova, V. V. Shupletsova, O. G. Khaziakhmatova [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: p. 118-119 (25 tit.)] | ||
| 330 | |a Migration, proliferation, and osteogenic differentiation of human adipose-derived (AD) multipotent mesenchymal stromal cells (MMSCs) during in vitro modeling of indirect contact with calcium phosphate (CP) or nanoparticles of synthetic hydroxyapatite (HA) have been studied. The results were registered with electrode (real-time cell analysis, RTCA) or visual (Cell-IQ) systems of long-term observation of cell cultures. Bulk specimens were use in a Cell-IQ® v2 MLF device as pure titanium substrates (10 Ч 10 Ч 1 mm3) covered by a CP relief (roughness index Ra = 2.4–4.4 µm) bilateral coating that was prepared by the micr-arc method from an aqueous solution of orthophosphoric acid (20 wt %), calcium carbonate (9 wt %), and synthetic HA (6 wt %). HA crystallites (1 mg/mL) were fabricated by mechanochemical synthesis and served as an irritant in RTCA investigation. The Cell-IQ system identified a 3.5- to 10-fold decrease in cell number at the interface with CP coatings with differing roughness during 14-day cell culturing. After 21 days, it was accompanied by a weak reduction of MMSC antigen expression (CD73, CD90, and CD105) as opposed to an increase in MMSC osteogenic differentiation and intercellular-matrix mineralization. In turn, HA nanodispersion reduced the speed of MMSC migration by 1.5 times (P < 0.001) during 25-h RTCA recording, which simulated cell invasion through the microporous membrane (8-µm diameter). Inhibition of migration and cell division with increased osteogenic differentiation of MMSCs has been suggested to be a possible effect of biodegradation products of synthetic CP materials. | ||
| 333 | |a Режим доступа: по договору с организацией-держателем ресурса | ||
| 338 | |b Российский научный фонд |d 16-15-10031 | ||
| 338 | |b Российский фонд фундаментальных исследований |d 15-03-07659 | ||
| 461 | |t Cell and Tissue Biology | ||
| 463 | |t Vol. 12, iss. 2 |v [P. 112–119] |d 2018 | ||
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a труды учёных ТПУ | |
| 610 | 1 | |a stromal cells of human adipose tissue | |
| 610 | 1 | |a motility | |
| 610 | 1 | |a trafficking | |
| 610 | 1 | |a membrane markers | |
| 610 | 1 | |a osteogenic differentiation | |
| 610 | 1 | |a hydroxylapatite | |
| 610 | 1 | |a calcium phosphate coating | |
| 610 | 1 | |a microtopography | |
| 610 | 1 | |a клетки | |
| 610 | 1 | |a жировые ткани | |
| 610 | 1 | |a мембранные маркеры | |
| 610 | 1 | |a дифференциация | |
| 610 | 1 | |a гидроксилапатиты | |
| 610 | 1 | |a покрытия | |
| 610 | 1 | |a фосфат кальция | |
| 610 | 1 | |a микрорельефы | |
| 701 | 1 | |a Livinova |b L. S. |g Larisa Sergeevna | |
| 701 | 1 | |a Shupletsova |b V. V. |g Valeria Vladimirovna | |
| 701 | 1 | |a Khaziakhmatova |b O. G. |g Olga Gennadjevna | |
| 701 | 1 | |a Yurova |b K. A. |g Kristina Alekseevna | |
| 701 | 1 | |a Malashchenko |b V. V. |g Vladimir Vladimirovich | |
| 701 | 1 | |a Melashchenko |b E. S. |g Elena Sergeevna | |
| 701 | 1 | |a Todosenko |b N. M. |g Nataljya Mikhaylovna | |
| 701 | 1 | |a Khlusova |b M. Yu. |g Marina Yurjevna | |
| 701 | 1 | |a Sharkeev |b Yu. P. |c physicist |c Professor of Tomsk Polytechnic University, Doctor of physical and mathematical sciences |f 1950- |g Yury Petrovich |3 (RuTPU)RU\TPU\pers\32228 |9 16228 | |
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| 701 | 1 | |a Sedelnikova |b M. B. |g Maria Borisovna | |
| 701 | 1 | |a Shunkin |b E. O. |g Egor Olegovich | |
| 701 | 1 | |a Khlusov |b I. A. |c biophysicist |c Professor of Tomsk Polytechnic University, doctor of medical Sciences |f 1963- |g Igor Albertovich |9 18225 | |
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