Members of the Uncultured Taxon OP1 ("Acetothermia") Predominate in the Microbial Community of an Alkaline Hot Spring at East-Tuvinian Upland; Microbiology; Vol. 87, iss. 6

Detalles Bibliográficos
Parent link:Microbiology
Vol. 87, iss. 6.— 2018.— [P. 783–795]
Autor Corporativo: Национальный исследовательский Томский политехнический университет Институт природных ресурсов Кафедра гидрогеологии, инженерной геологии и гидрогеоэкологии Научно-образовательный центр "Вода" Проблемная научно-исследовательская лаборатория гидрогеохимии
Outros autores: Korzhenkov A. A., Teplyuk A. V., Lebedinskii A. V., Khvaschevskaya A. A. Albina Anatolievna, Kopylova Yu. G. Yuliya Grigorievna, Arakchaa K. D., Golyshin P. N. Peter, Lunev E. A., Golyshina O. V., Kublanov I. V. Ilya, Toshchakov S. V., Gavrilov S. N.
Summary:Title screen
The thermophilic microbial community of a hydrotherm at East-Tuvinian upland is described. High-throughput profiling of the 16S rRNA gene and sequencing of the complete metagenome were used to characterize the phylogenetic and metabolic diversity of the microbial community of the Dikij Arjaan alkaline thermal spring of Ush-Bel'dir mineral water deposit in Tyva, Russia. Members of the uncultured phylum "Acetothermia" (candidate division OP1) predominated in the microbial community of the spring. While this phylum has already been detected in many thermal ecosystems, it never formed the main component of their microbial communities. Apart from "Acetothermia", members of Nitrospirae and Chloroflexi comprised a significant part of the microbial community. Comparative analysis of our geochemical data on the spring and the previously published data on in silico reconstruction of "Acetothermia" metabolism led us to the suggestion that carbonate and bicarbonate can serve as the major carbon sources for the dominant bacterial group and that "Acetothermia" act as the primary producers in this ecosystem. Analysis of the functional genes also revealed the presence of physiological groups of denitrifyers, iron reducers, carboxydotrophs and diazotrophs. This is the first report on a thermophilic microbial community of a hydrotherm from the southwestern extremity of the Baikal rift zone. Moreover, microbial communities with predominance of uncultured "Acetothermia" have not been reported previously in terrestrial hydrotherms.
Режим доступа: по договору с организацией-держателем ресурса
Idioma:inglés
Publicado: 2018
Subjects:
Acceso en liña:https://doi.org/10.1134/S0026261718060115
Formato: xMaterials Electrónico Capítulo de libro
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=663884

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200 1 |a Members of the Uncultured Taxon OP1 ("Acetothermia") Predominate in the Microbial Community of an Alkaline Hot Spring at East-Tuvinian Upland  |f A. A. Korzhenkov, A. V. Teplyuk, A. V. Lebedinskii [et al.] 
203 |a Text  |c electronic 
300 |a Title screen 
320 |a [References: p. 794-795 (38 tit.)] 
330 |a The thermophilic microbial community of a hydrotherm at East-Tuvinian upland is described. High-throughput profiling of the 16S rRNA gene and sequencing of the complete metagenome were used to characterize the phylogenetic and metabolic diversity of the microbial community of the Dikij Arjaan alkaline thermal spring of Ush-Bel'dir mineral water deposit in Tyva, Russia. Members of the uncultured phylum "Acetothermia" (candidate division OP1) predominated in the microbial community of the spring. While this phylum has already been detected in many thermal ecosystems, it never formed the main component of their microbial communities. Apart from "Acetothermia", members of Nitrospirae and Chloroflexi comprised a significant part of the microbial community. Comparative analysis of our geochemical data on the spring and the previously published data on in silico reconstruction of "Acetothermia" metabolism led us to the suggestion that carbonate and bicarbonate can serve as the major carbon sources for the dominant bacterial group and that "Acetothermia" act as the primary producers in this ecosystem. Analysis of the functional genes also revealed the presence of physiological groups of denitrifyers, iron reducers, carboxydotrophs and diazotrophs. This is the first report on a thermophilic microbial community of a hydrotherm from the southwestern extremity of the Baikal rift zone. Moreover, microbial communities with predominance of uncultured "Acetothermia" have not been reported previously in terrestrial hydrotherms. 
333 |a Режим доступа: по договору с организацией-держателем ресурса 
338 |b Российский фонд фундаментальных исследований  |d 16-54-76022 
338 |b Российский фонд фундаментальных исследований  |d 16-54-10072 
461 |t Microbiology 
463 |t Vol. 87, iss. 6  |v [P. 783–795]  |d 2018 
610 1 |a электронный ресурс 
610 1 |a труды учёных ТПУ 
610 1 |a uncultured bacteria 
610 1 |a OP1 
610 1 |a Acetothermia 
610 1 |a new acetyl-CoA synthases 
610 1 |a terrestrial hydrotherms 
610 1 |a EastTuvinian Upland 
610 1 |a бактерии 
610 1 |a гидротермы 
610 1 |a горячие источники 
701 1 |a Korzhenkov  |b A. A. 
701 1 |a Teplyuk  |b A. V. 
701 1 |a Lebedinskii  |b A. V. 
701 1 |a Khvaschevskaya  |b A. A.  |c hydrogeologist  |c Associate Professor of Tomsk Polytechnic University, Candidate of geological and mineralogical sciences  |f 1969-  |g Albina Anatolievna  |3 (RuTPU)RU\TPU\pers\30953 
701 1 |a Kopylova  |b Yu. G.  |c hydrogeologist  |c Associate Professor of Tomsk Polytechnic University, Candidate of geological and mineralogical sciences  |f 1941-  |g Yuliya Grigorievna  |3 (RuTPU)RU\TPU\pers\33262 
701 1 |a Arakchaa  |b K. D. 
701 1 |a Golyshin  |b P. N.  |g Peter 
701 1 |a Lunev  |b E. A. 
701 1 |a Golyshina  |b O. V. 
701 1 |a Kublanov  |b I. V.  |g Ilya 
701 1 |a Toshchakov  |b S. V. 
701 1 |a Gavrilov  |b S. N. 
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