The Growth of 3T3 Fibroblasts on PHB, PLA and PHB/PLA Blend Films at Different Stages of Their Biodegradation In Vitro; Polymers; Vol. 13, iss. 1
| Parent link: | Polymers Vol. 13, iss. 1.— 2021.— [108, 24 p.] |
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| Korporacja: | |
| Kolejni autorzy: | , , , , , , , , , , , , , |
| Streszczenie: | Title screen Over the past century there was a significant development and extensive application of biodegradable and biocompatible polymers for their biomedical applications. This research investigates the dynamic change in properties of biodegradable polymers: poly(3-hydroxybutyrate (PHB), poly-l-lactide (PLA), and their 50:50 blend (PHB/PLA)) during their hydrolytic non-enzymatic (in phosphate buffered saline (PBS), at pH = 7.4, 37 °C) and enzymatic degradation (in PBS supplemented with 0.25 mg/mL pancreatic lipase). 3T3 fibroblast proliferation on the polymer films experiencing different degradation durations was also studied. Enzymatic degradation significantly accelerated the degradation rate of polymers compared to non-enzymatic hydrolytic degradation, whereas the seeding of 3T3 cells on the polymer films accelerated only the PLA molecular weight loss. Surprisingly, the immiscible nature of PHB/PLA blend (showed by differential scanning calorimetry) led to a slower and more uniform enzymatic degradation in comparison with pure polymers, PHB and PLA, which displayed a two-stage degradation process. PHB/PLA blend also displayed relatively stable cell viability on films upon exposure to degradation of different durations, which was associated with the uneven distribution of cells on polymer films. Thus, the obtained data are of great benefit for designing biodegradable scaffolds based on polymer blends for tissue engineering. |
| Język: | angielski |
| Wydane: |
2021
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| Hasła przedmiotowe: | |
| Dostęp online: | https://doi.org/10.3390/polym13010108 |
| Format: | MixedMaterials Elektroniczne Rozdział |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=663880 |
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| 200 | 1 | |a The Growth of 3T3 Fibroblasts on PHB, PLA and PHB/PLA Blend Films at Different Stages of Their Biodegradation In Vitro |f V. A. Zhuikov, E. A. Akoulina, D. V. Chesnokova [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: 48 tit.] | ||
| 330 | |a Over the past century there was a significant development and extensive application of biodegradable and biocompatible polymers for their biomedical applications. This research investigates the dynamic change in properties of biodegradable polymers: poly(3-hydroxybutyrate (PHB), poly-l-lactide (PLA), and their 50:50 blend (PHB/PLA)) during their hydrolytic non-enzymatic (in phosphate buffered saline (PBS), at pH = 7.4, 37 °C) and enzymatic degradation (in PBS supplemented with 0.25 mg/mL pancreatic lipase). 3T3 fibroblast proliferation on the polymer films experiencing different degradation durations was also studied. Enzymatic degradation significantly accelerated the degradation rate of polymers compared to non-enzymatic hydrolytic degradation, whereas the seeding of 3T3 cells on the polymer films accelerated only the PLA molecular weight loss. Surprisingly, the immiscible nature of PHB/PLA blend (showed by differential scanning calorimetry) led to a slower and more uniform enzymatic degradation in comparison with pure polymers, PHB and PLA, which displayed a two-stage degradation process. PHB/PLA blend also displayed relatively stable cell viability on films upon exposure to degradation of different durations, which was associated with the uneven distribution of cells on polymer films. Thus, the obtained data are of great benefit for designing biodegradable scaffolds based on polymer blends for tissue engineering. | ||
| 461 | |t Polymers | ||
| 463 | |t Vol. 13, iss. 1 |v [108, 24 p.] |d 2021 | ||
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a труды учёных ТПУ | |
| 610 | 1 | |a poly(3-hydroxybutyrate) | |
| 610 | 1 | |a poly-l-lactide | |
| 610 | 1 | |a polymer blend | |
| 610 | 1 | |a biodegradation | |
| 610 | 1 | |a hydrolysis | |
| 610 | 1 | |a pancreatic lipase | |
| 610 | 1 | |a 3T3 fibroblasts | |
| 610 | 1 | |a nanoparticles | |
| 610 | 1 | |a полимерные смеси | |
| 610 | 1 | |a биоразложение | |
| 610 | 1 | |a гидролиз | |
| 701 | 1 | |a Zhuikov |b V. A. |g Vsevolod | |
| 701 | 1 | |a Akoulina |b E. A. |g Elizaveta | |
| 701 | 1 | |a Chesnokova |b D. V. |g Dariana | |
| 701 | 0 | |a Wenhao You | |
| 701 | 1 | |a Makhina |b T. K. |g Tatiana | |
| 701 | 1 | |a Demyanova |b I. V. |g Irina | |
| 701 | 1 | |a Zhuikova |b Yu. V. |g Yuliya | |
| 701 | 1 | |a Voinova |b V. V. |g Vera | |
| 701 | 1 | |a Belishev |b N. V. |g Nikita | |
| 701 | 1 | |a Surmenev |b R. A. |c physicist |c Associate Professor of Tomsk Polytechnic University, Senior researcher, Candidate of physical and mathematical sciences |f 1982- |g Roman Anatolievich |3 (RuTPU)RU\TPU\pers\31885 |9 15957 | |
| 701 | 1 | |a Surmeneva |b M. A. |c specialist in the field of material science |c engineer-researcher of Tomsk Polytechnic University, Associate Scientist |f 1984- |g Maria Alexandrovna |3 (RuTPU)RU\TPU\pers\31894 |9 15966 | |
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