Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa; Molecules; Vol. 24, iss. 20

Bibliografske podrobnosti
Parent link:	Molecules Vol. 24, iss. 20.— 2019.— [3680, 15 p.]
Korporativna značnica:	Национальный исследовательский Томский политехнический университет Исследовательская школа химических и биомедицинских технологий
Drugi avtorji:	Khlusov I. A. Igor Albertovich, Avdeeva E. Elena, Schupletsova V. V. Valeriya, Khaziakhmatova O. G. Olga Gennadjevna, Litvinova L. S., Porokhova E. Ekaterina, Reshetov Ya. E. Yaroslav Evgenjevich, Zvereva I. Irina, Mushtovatova Lyudmila L., Karpova M. R. Mariya Rostislavovna, Gurjev A. M. Artem Mikhaylovich, Sukhodolo I. V. Irina Vladimirovna, Belousov M. V. Mikhail Valerievich
Izvleček:	Title screen The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC > F-SC) at doses of 10-50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications.
Jezik:	angleščina
Izdano:	2019
Teme:	труды учёных ТПУ электронный ресурс Saussurea controversa DC (Asteraceae) polysaccharides flavonoids human adipose-derived multipotent mesenchymal stromal cells cytotoxicity alizarin red staining полисахариды флавоноиды клетки жировые ткани цитотоксичность окрашивание антибактериальная активность
Online dostop:	https://doi.org/10.3390/molecules24203680
Format:	MixedMaterials Elektronski Book Chapter
KOHA link:	https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=663842