Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa; Molecules; Vol. 24, iss. 20
| Parent link: | Molecules Vol. 24, iss. 20.— 2019.— [3680, 15 p.] |
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| Müşterek Yazar: | |
| Diğer Yazarlar: | , , , , , , , , , , , , |
| Özet: | Title screen The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC > F-SC) at doses of 10-50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications. |
| Dil: | İngilizce |
| Baskı/Yayın Bilgisi: |
2019
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| Konular: | |
| Online Erişim: | https://doi.org/10.3390/molecules24203680 |
| Materyal Türü: | Elektronik Kitap Bölümü |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=663842 |
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| 200 | 1 | |a Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa |f I. A. Khlusov, E. Avdeeva, V. V. Schupletsova [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: 45 tit.] | ||
| 330 | |a The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC > F-SC) at doses of 10-50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications. | ||
| 461 | |t Molecules | ||
| 463 | |t Vol. 24, iss. 20 |v [3680, 15 p.] |d 2019 | ||
| 610 | 1 | |a труды учёных ТПУ | |
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a Saussurea controversa DC (Asteraceae) | |
| 610 | 1 | |a polysaccharides | |
| 610 | 1 | |a flavonoids | |
| 610 | 1 | |a human adipose-derived multipotent mesenchymal stromal cells | |
| 610 | 1 | |a cytotoxicity | |
| 610 | 1 | |a alizarin red staining | |
| 610 | 1 | |a полисахариды | |
| 610 | 1 | |a флавоноиды | |
| 610 | 1 | |a клетки | |
| 610 | 1 | |a жировые ткани | |
| 610 | 1 | |a цитотоксичность | |
| 610 | 1 | |a окрашивание | |
| 610 | 1 | |a антибактериальная активность | |
| 701 | 1 | |a Khlusov |b I. A. |c biophysicist |c Professor of Tomsk Polytechnic University, doctor of medical Sciences |f 1963- |g Igor Albertovich |3 (RuTPU)RU\TPU\pers\34907 |9 18225 | |
| 701 | 1 | |a Avdeeva |b E. |g Elena | |
| 701 | 1 | |a Schupletsova |b V. V. |g Valeriya | |
| 701 | 1 | |a Khaziakhmatova |b O. G. |g Olga Gennadjevna | |
| 701 | 1 | |a Litvinova |b L. S. | |
| 701 | 1 | |a Porokhova |b E. |g Ekaterina | |
| 701 | 1 | |a Reshetov |b Ya. E. |g Yaroslav Evgenjevich | |
| 701 | 1 | |a Zvereva |b I. |g Irina | |
| 701 | 1 | |a Mushtovatova |b Lyudmila |g L. | |
| 701 | 1 | |a Karpova |b M. R. |g Mariya Rostislavovna | |
| 701 | 1 | |a Gurjev |b A. M. |g Artem Mikhaylovich | |
| 701 | 1 | |a Sukhodolo |b I. V. |g Irina Vladimirovna | |
| 701 | 1 | |a Belousov |b M. V. |c chemist |c Professor of Tomsk Polytechnic University, Doctor of Pharmaceutical Sciences |f 1963- |g Mikhail Valerievich |3 (RuTPU)RU\TPU\pers\45418 |9 21924 | |
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