Development and validation of HPLC-UV method for quantitation of a new antithrombotic drug in rat plasma and its application to pharmacokinetic studies; Journal of Chromatography B; Vol. 1160

Development and validation of HPLC-UV method for quantitation of a new antithrombotic drug in rat plasma and its application to pharmacokinetic studies; Journal of Chromatography B; Vol. 1160

التفاصيل البيبلوغرافية
Parent link:Journal of Chromatography B
Vol. 1160.— 2020.— [122382, 6 p.]
مؤلف مشترك: Национальный исследовательский Томский политехнический университет Инженерная школа природных ресурсов Отделение химической инженерии
مؤلفون آخرون: Leonov K. A. Klim Andreevich, Vishenkova D. A. Dariya Aleksandrovna, Lipskikh O. I. Olga Ivanovna, Pustovoitov A. V. Andrey Vladimirovich, Bakibaev A. A. Abdigali Abdimanapovich
الملخص:Title screen
A selective and sensitive procedure for quantitation of a new antithrombotic drug (GRS) in rat plasma was developed and validated using an HPLC-UV. The method was validated according to recommendations of the FDA, EMA in terms of selectivity, linearity, accuracy, precision, recovery, matrix effect, stability, and carry-over. The preparation of the biological sample included liquid-liquid extraction with acetonitrile, separation of water-organic mixture with inorganic salts, organic phase clean-up with a sorbent (QuEChERS method), its evaporation to dryness and reconstitution of the residue with A:B eluents mixture (1:1). The chromatographic separations were performed on a micro-column 75 × 2 mm, 5 μm particle size sorbent ProntoSIL 120-5-C18 AQ. The flowrate was of 0.15 ml/min, detector wavelength was set at 360 nm for GRS and at 230 nm for papaverine (IS). It was found that GRS recovery from rat plasma is 94%, the response linearity is in the range of 10 to 1000 ng ml−1. The accuracy values for intra-day determination were of 93.2 to 101.8%, for inter-day determination were of 91.2 to 102.2%, coefficient of variation for intra- and inter-day precision did not exceed 4.1 to 9.3%. The application of the method was shown in pharmacokinetic studies of GRS in rats at a dose of 20 mg kg−1.
Режим доступа: по договору с организацией-держателем ресурса
اللغة:الإنجليزية
منشور في: 2020
الموضوعات:
электронный ресурс
труды учёных ТПУ
HPLC-UV
pharmacokinetic studies
antithrombotic drug
rat plasma
фармакологические препараты
антитромботические средства
الوصول للمادة أونلاين:https://doi.org/10.1016/j.jchromb.2020.122382
التنسيق: الكتروني فصل الكتاب
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=663457

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