Bone-Like Multilevel Calcium Phosphate Coating Modulates an Interaction of Mesenchymal Stem Cells and Tumor Cells
| Parent link: | AIP Conference Proceedings Vol. 2167 : Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019 (AMHS'19).— 2019.— [020215, 5 p.] |
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| Andre forfattere: | , , , , , , , , , , |
| Summary: | Title screen Modern biomaterial biocompatibility research focuses the biomaterial hierarchic effects on multipotent mesenchymal stromal cell (MMSC) behavior (that occur at the nano-, micro- and macroscales) because MMSCs are the fundamental units that produce/regenerate bone tissue. Leukemia initiation and progression are connected with a disfunction of health cell microenvironment and MMSCs. Continuous monitoring of MMSC and tumor cell interaction is a promising tool for oncology, cellular biology, biotechnology and environmental research. The aim was to investigate a modulation of in vitro interaction of human MMSCs and leukemic T lymphoblast-like cells (Jurkat T cells) caused by micro-arc multilevel calcium phosphate (CP) coating with the help of Cell-IQ and RTCA advanced tools for continuous monitoring. An average velocity of cell division (AVCD) of human adipose-derived MMSCs (hAMMSCs) contacted in vitro with allogenic Jurkat line of human leukemic T lymphoblasts (Jurkat T cells) was studied by means of Cell-IQ v2 MLF integrated phase-contrast microscopic platform for real-time surveillance imaging of living cells. Both 50 [mu]L suspensions (5×104 viable karyocytes) of the CD73CD90CD105+ adherent cells and Jurkat T cells were applied into the center of the well of 12-well plastic plates for 7 days at 100% humidity in a 5% CO2 atmosphere at 37°C until a monolayer formation. A nutrient medium was once replaced. To determine cell invasion (chemotactic motility) through 8 [mu]m pores the real-time cell analysis (RTCA DP Analyzer) with the CIM-plate was used. AVCD of fibroblast-like adherent hAMMSCs was 0.27-0.63 divisions/h. CP coating diminished significantly the percent of dividing hAMMSCs contacted with leukemic Jurkat T cells. RTCA system showed significant hAMMSC invasion towards tumor cells and not vice versa. For all this, cellular interaction led to increasing viability of Jurkat T cells and decreasing hAMMSC viability. Thus, tumor Jurkat T cells could control a fate of health hAMMSCs and promote stromal microenvironment for survivability of tumor clones by means of secretable molecular products. Multilevel micro-arc CP coating forms bonelike inorganic structure that is capable to modulate in vitro interaction of human MMSCs and leukemic T lymphoblasts. The results obtained may be useful for replacement surgery applications of orthopedic implants in cancer patients. Режим доступа: по договору с организацией-держателем ресурса |
| Sprog: | engelsk |
| Udgivet: |
2019
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| Fag: | |
| Online adgang: | https://doi.org/10.1063/1.5132082 |
| Format: | Electronisk Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=661513 |
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| 200 | 1 | |a Bone-Like Multilevel Calcium Phosphate Coating Modulates an Interaction of Mesenchymal Stem Cells and Tumor Cells |f V. V. Malashchenko [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: 12 tit.] | ||
| 330 | |a Modern biomaterial biocompatibility research focuses the biomaterial hierarchic effects on multipotent mesenchymal stromal cell (MMSC) behavior (that occur at the nano-, micro- and macroscales) because MMSCs are the fundamental units that produce/regenerate bone tissue. Leukemia initiation and progression are connected with a disfunction of health cell microenvironment and MMSCs. Continuous monitoring of MMSC and tumor cell interaction is a promising tool for oncology, cellular biology, biotechnology and environmental research. The aim was to investigate a modulation of in vitro interaction of human MMSCs and leukemic T lymphoblast-like cells (Jurkat T cells) caused by micro-arc multilevel calcium phosphate (CP) coating with the help of Cell-IQ and RTCA advanced tools for continuous monitoring. An average velocity of cell division (AVCD) of human adipose-derived MMSCs (hAMMSCs) contacted in vitro with allogenic Jurkat line of human leukemic T lymphoblasts (Jurkat T cells) was studied by means of Cell-IQ v2 MLF integrated phase-contrast microscopic platform for real-time surveillance imaging of living cells. Both 50 [mu]L suspensions (5×104 viable karyocytes) of the CD73CD90CD105+ adherent cells and Jurkat T cells were applied into the center of the well of 12-well plastic plates for 7 days at 100% humidity in a 5% CO2 atmosphere at 37°C until a monolayer formation. | ||
| 330 | |a A nutrient medium was once replaced. To determine cell invasion (chemotactic motility) through 8 [mu]m pores the real-time cell analysis (RTCA DP Analyzer) with the CIM-plate was used. AVCD of fibroblast-like adherent hAMMSCs was 0.27-0.63 divisions/h. CP coating diminished significantly the percent of dividing hAMMSCs contacted with leukemic Jurkat T cells. RTCA system showed significant hAMMSC invasion towards tumor cells and not vice versa. For all this, cellular interaction led to increasing viability of Jurkat T cells and decreasing hAMMSC viability. Thus, tumor Jurkat T cells could control a fate of health hAMMSCs and promote stromal microenvironment for survivability of tumor clones by means of secretable molecular products. Multilevel micro-arc CP coating forms bonelike inorganic structure that is capable to modulate in vitro interaction of human MMSCs and leukemic T lymphoblasts. The results obtained may be useful for replacement surgery applications of orthopedic implants in cancer patients. | ||
| 333 | |a Режим доступа: по договору с организацией-держателем ресурса | ||
| 461 | 1 | |0 (RuTPU)RU\TPU\network\4816 |t AIP Conference Proceedings | |
| 463 | 1 | |0 (RuTPU)RU\TPU\network\31884 |t Vol. 2167 : Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019 (AMHS'19) |o Proceedings of the International Conference, 1–5 October 2019, Tomsk, Russia |f National Research Tomsk Polytechnic University (TPU) ; Institute of Strength Physics and Materials Science SB RAS (Russia) ; eds. V. E. Panin ; S. G. Psakhie ; V. M. Fomin |v [020215, 5 p.] |d 2019 | |
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