Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models
| Parent link: | Oxidative Medicine and Cellular Longevity Vol. 2019.— 2019.— [4528241, 12 p.] |
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| Ente Autore: | |
| Altri autori: | , , , , , , , , |
| Riassunto: | Title screen During the last 3 decades, there has been a slow advance to obtain new treatments for malignant melanoma that improve patient survival. In this work, we present a systematic study focused on the antiproliferative and antitumour effect of AgNPs. These nanoparticles are fully characterized, are coated with polyvinylpyrrolidone (PVP), and have an average size of and a metallic silver content of 1.2% wt. Main changes on cell viability, induction of apoptosis and necrosis, and ROS generation were found on B16-F10 cells after six hours of exposure to AgNPs () or Cisplatin (). Despite the similar response for both AgNPs and Cisplatin on antiproliferative potency (cellular viability of and ) and ROS production ( and ), significantly different cell death pathways were triggered. While AgNPs induce only apoptosis (), Cisplatin induces apoptosis and necrosis at the same rate ( and , respectively). In addition to their antiproliferative activity, in vivo experiments showed that treatments of 3, 6, and 12 mg/kg of AgNPs elicit a survival rate almost 4 times higher () compared with the survival rate obtained with Cisplatin (2 mg/kg). Furthermore, the survivor mice treated with AgNPs do not show genotoxic damage determined by micronuclei frequency quantification on peripheral blood cells. These results exhibit the remarkable antitumour activity of a nongenotoxic AgNP formulation and constitute the first advance toward the application of these AgNPs for melanoma treatment, which could considerably reduce adverse effects provoked by currently applied chemotherapeutics. |
| Pubblicazione: |
2019
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| Soggetti: | |
| Accesso online: | https://doi.org/10.1155/2019/4528241 |
| Natura: | Elettronico Capitolo di libro |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=661244 |
| Riassunto: | Title screen During the last 3 decades, there has been a slow advance to obtain new treatments for malignant melanoma that improve patient survival. In this work, we present a systematic study focused on the antiproliferative and antitumour effect of AgNPs. These nanoparticles are fully characterized, are coated with polyvinylpyrrolidone (PVP), and have an average size of and a metallic silver content of 1.2% wt. Main changes on cell viability, induction of apoptosis and necrosis, and ROS generation were found on B16-F10 cells after six hours of exposure to AgNPs () or Cisplatin (). Despite the similar response for both AgNPs and Cisplatin on antiproliferative potency (cellular viability of and ) and ROS production ( and ), significantly different cell death pathways were triggered. While AgNPs induce only apoptosis (), Cisplatin induces apoptosis and necrosis at the same rate ( and , respectively). In addition to their antiproliferative activity, in vivo experiments showed that treatments of 3, 6, and 12 mg/kg of AgNPs elicit a survival rate almost 4 times higher () compared with the survival rate obtained with Cisplatin (2 mg/kg). Furthermore, the survivor mice treated with AgNPs do not show genotoxic damage determined by micronuclei frequency quantification on peripheral blood cells. These results exhibit the remarkable antitumour activity of a nongenotoxic AgNP formulation and constitute the first advance toward the application of these AgNPs for melanoma treatment, which could considerably reduce adverse effects provoked by currently applied chemotherapeutics. |
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| DOI: | 10.1155/2019/4528241 |