High-Performance Ultrasonic Tweezers for Manipulation of Motile and Still Single Cells in a Droplet; Ultrasound in Medicine & Biology; Vol. 45, iss. 11

High-Performance Ultrasonic Tweezers for Manipulation of Motile and Still Single Cells in a Droplet; Ultrasound in Medicine & Biology; Vol. 45, iss. 11

Detalles Bibliográficos
Parent link:Ultrasound in Medicine & Biology
Vol. 45, iss. 11.— 2019.— [P. 3018-3027]
Autor Corporativo: Национальный исследовательский Томский политехнический университет Инженерная школа неразрушающего контроля и безопасности Отделение электронной инженерии
Otros Autores: Liu Qingyang, Minin O. V. Oleg Vladilenovich, Minin I. V. Igor Vladilenovich
Sumario:Title screen
The manipulation of motile and still single cells with the simultaneous features of selective trapping, 3-D path free transport, position-controllable release and little heat damage has been a significant challenge. We developed an ultrasonic method for capturing motile and still single cells with the aforementioned features in a droplet. During manipulation, a micromanipulation probe (MMP), which vibrated linearly with a trajectory parallel to a silicon substrate, was immersed in the droplet and was not in contact with the substrate. Motile and still single cells, such as Chattonella marina with a length of 30-50 μm and yeast cells with a diameter of 3-10 μm, at the interface between the droplet and substrate were selectively sucked onto the vibrating MMP and transported via a 3-D route inside the droplet by moving the MMP (or the device). The MMP and captured single cells were in contact, making the release position controllable. The measured temperature rise of the MMP was <0.1°C; thus, it is competitive for the manipulation of biological samples. Finite-element analyses revealed that the contact-type capture was due to acoustic radiation force generated by the ultrasonic field around the vibrating MMP. The dependence of the capture capability and working frequency bandwidth on the working conditions was investigated experimentally.
Режим доступа: по договору с организацией-держателем ресурса
Lenguaje:inglés
Publicado: 2019
Materias:
электронный ресурс
труды учёных ТПУ
cell manipulation
tweezers
ultrasound
ультразвук
Acceso en línea:https://doi.org/10.1016/j.ultrasmedbio.2019.08.002
Formato: Electrónico Capítulo de libro
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=661230

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