High-Performance Ultrasonic Tweezers for Manipulation of Motile and Still Single Cells in a Droplet; Ultrasound in Medicine & Biology; Vol. 45, iss. 11

High-Performance Ultrasonic Tweezers for Manipulation of Motile and Still Single Cells in a Droplet; Ultrasound in Medicine & Biology; Vol. 45, iss. 11

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Parent link:Ultrasound in Medicine & Biology
Vol. 45, iss. 11.— 2019.— [P. 3018-3027]
Korporativní autor: Национальный исследовательский Томский политехнический университет Инженерная школа неразрушающего контроля и безопасности Отделение электронной инженерии
Další autoři: Liu Qingyang, Minin O. V. Oleg Vladilenovich, Minin I. V. Igor Vladilenovich
Shrnutí:Title screen
The manipulation of motile and still single cells with the simultaneous features of selective trapping, 3-D path free transport, position-controllable release and little heat damage has been a significant challenge. We developed an ultrasonic method for capturing motile and still single cells with the aforementioned features in a droplet. During manipulation, a micromanipulation probe (MMP), which vibrated linearly with a trajectory parallel to a silicon substrate, was immersed in the droplet and was not in contact with the substrate. Motile and still single cells, such as Chattonella marina with a length of 30-50 μm and yeast cells with a diameter of 3-10 μm, at the interface between the droplet and substrate were selectively sucked onto the vibrating MMP and transported via a 3-D route inside the droplet by moving the MMP (or the device). The MMP and captured single cells were in contact, making the release position controllable. The measured temperature rise of the MMP was <0.1°C; thus, it is competitive for the manipulation of biological samples. Finite-element analyses revealed that the contact-type capture was due to acoustic radiation force generated by the ultrasonic field around the vibrating MMP. The dependence of the capture capability and working frequency bandwidth on the working conditions was investigated experimentally.
Режим доступа: по договору с организацией-держателем ресурса
Jazyk:angličtina
Vydáno: 2019
Témata:
электронный ресурс
труды учёных ТПУ
cell manipulation
tweezers
ultrasound
ультразвук
On-line přístup:https://doi.org/10.1016/j.ultrasmedbio.2019.08.002
Médium: Elektronický zdroj Kapitola
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=661230

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