Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis; Sensors; Vol. 19, iss. 9

Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis; Sensors; Vol. 19, iss. 9

Manylion Llyfryddiaeth
Parent link:	Sensors Vol. 19, iss. 9.— 2019.— [2103, 10 p.]
Awdur Corfforaethol:	Национальный исследовательский Томский политехнический университет Инженерная школа природных ресурсов Отделение химической инженерии
Awduron Eraill:	Khristunova E. Ekaterina, Korotkova E. I. Elena Ivanovna, Kratochvil B. Bohumil, Barek J. Jiri, Dorozhko E. V. Elena Vladimirovna, Vyskocil V. Vlastimil, Plotnikov E. V. Evgeny Vladimirovich, Voronova O. A. Olesya Aleksandrovna, Sidelnikov V. S. Vladimir Sergeevich
Crynodeb:	Title screen A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (Ab@AgNP). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold–carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their Ab@AgNP. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for Ab@AgNP preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL?1 to 1600 IU·mL?1, with a detection limit of 50 IU·mL?1. The coefficient of determination (r2) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.
Iaith:	Saesneg
Cyhoeddwyd:	2019
Pynciau:	труды учёных ТПУ электронный ресурс bioconjugates silver nanoparticles antibodies tick-borne encephalitis virus gold–carbon composite electrode electrochemical immunosensor биоконъюгаты наночастицы антитела вирусы
Mynediad Ar-lein:	http://earchive.tpu.ru/handle/11683/57327 https://doi.org/10.3390/s19092103
Fformat:	Electronig Pennod Llyfr
KOHA link:	https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=660602

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