Phototoxicity of flavoprotein miniSOG induced by bioluminescence resonance energy transfer in genetically encoded system NanoLuc-miniSOG is comparable with its LED-excited phototoxicity; Journal of Photochemistry and Photobiology B: Biology; Vol. 188
| Parent link: | Journal of Photochemistry and Photobiology B: Biology Vol. 188.— 2018.— [P. 107-115] |
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| সংস্থা লেখক: | |
| অন্যান্য লেখক: | , , , , |
| সংক্ষিপ্ত: | Title screen Photodynamic therapy (PDT) is a clinical, minimally invasive method for destroying cancer cells in the presence of a photosensitizer, oxygen, and a light source. The main obstacle for the PDT treatment of deep tumors is a strong reduction of the excitation light intensity as a result of its refraction, reflection, and absorption by biological tissues. Internal light sources based on bioluminescence resonance energy transfer can be a solution of this problem. Here we show that luciferase NanoLuc being expressed as a fusion protein with phototoxic flavoprotein miniSOG in cancer cells in the presence of furimazine (highly specific NanoLuc substrate) induces a photodynamic effect of miniSOG comparable with its LED-excited (Light Emitting Diode) phototoxicity. Luminescence systems based on furimazine and hybrid protein NanoLuc-miniSOG targeted to mitochondria or cellular membranes possess the similar energy transfer efficiencies and similar BRET-induced cytotoxic effects on cancer cells, though the mechanisms of BRET-induced cell death are different. As the main components of the proposed system for BRET-mediated PDT are genetically encoded (luciferase and phototoxic protein), this system can potentially be delivered to any site in the organism and thus may be considered as a promising approach for simultaneous delivery of light source and photosensitizer in deep-lying tumors and metastasis anywhere in the body. Режим доступа: по договору с организацией-держателем ресурса |
| ভাষা: | ইংরেজি |
| প্রকাশিত: |
2018
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://doi.org/10.1016/j.jphotobiol.2018.09.006 |
| বিন্যাস: | MixedMaterials বৈদ্যুতিক গ্রন্থের অধ্যায় |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=658876 |
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| 200 | 1 | |a Phototoxicity of flavoprotein miniSOG induced by bioluminescence resonance energy transfer in genetically encoded system NanoLuc-miniSOG is comparable with its LED-excited phototoxicity |f G. M. Proshkina [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: p. 114-115 (54 tit.)] | ||
| 330 | |a Photodynamic therapy (PDT) is a clinical, minimally invasive method for destroying cancer cells in the presence of a photosensitizer, oxygen, and a light source. The main obstacle for the PDT treatment of deep tumors is a strong reduction of the excitation light intensity as a result of its refraction, reflection, and absorption by biological tissues. Internal light sources based on bioluminescence resonance energy transfer can be a solution of this problem. Here we show that luciferase NanoLuc being expressed as a fusion protein with phototoxic flavoprotein miniSOG in cancer cells in the presence of furimazine (highly specific NanoLuc substrate) induces a photodynamic effect of miniSOG comparable with its LED-excited (Light Emitting Diode) phototoxicity. Luminescence systems based on furimazine and hybrid protein NanoLuc-miniSOG targeted to mitochondria or cellular membranes possess the similar energy transfer efficiencies and similar BRET-induced cytotoxic effects on cancer cells, though the mechanisms of BRET-induced cell death are different. As the main components of the proposed system for BRET-mediated PDT are genetically encoded (luciferase and phototoxic protein), this system can potentially be delivered to any site in the organism and thus may be considered as a promising approach for simultaneous delivery of light source and photosensitizer in deep-lying tumors and metastasis anywhere in the body. | ||
| 333 | |a Режим доступа: по договору с организацией-держателем ресурса | ||
| 461 | 1 | |t Journal of Photochemistry and Photobiology B: Biology | |
| 463 | 1 | |t Vol. 188 |v [P. 107-115] |d 2018 | |
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a труды учёных ТПУ | |
| 610 | 1 | |a miniSOG | |
| 610 | 1 | |a Bioluminescence resonance energy transfer (BRET) | |
| 610 | 1 | |a BRET-mediated phototoxicity | |
| 610 | 1 | |a BRET efficiency | |
| 610 | 1 | |a NanoLuc | |
| 610 | 1 | |a передача энергии | |
| 610 | 1 | |a резонанс | |
| 610 | 1 | |a биолюминесценция | |
| 701 | 1 | |a Proshkina |b G. M. |g Galina Mikhaylovna | |
| 701 | 1 | |a Shramova |b E. I. |g Elena Ivanovna | |
| 701 | 1 | |a Shilova |b O. N. |g Olga Nikolaevna | |
| 701 | 1 | |a Ryabova |b A. V. |g Anastasiya Vladimirovna | |
| 701 | 1 | |a Deev |b S. M. |c biologist |c the expert of Tomsk Polytechnic University, doctor of biological Sciences |g Sergey Mikhaylovich |3 (RuTPU)RU\TPU\pers\39299 | |
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| 856 | 4 | |u https://doi.org/10.1016/j.jphotobiol.2018.09.006 | |
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