Lentiviral gene delivery to plasmolipin-expressing cells using Mus caroli endogenous retrovirus envelope protein

Lentiviral gene delivery to plasmolipin-expressing cells using Mus caroli endogenous retrovirus envelope protein

Bibliographic Details
Parent link:	Biochimie Vol. 142.— 2017.— [P. 226-233]
Corporate Author:	Национальный исследовательский Томский политехнический университет (ТПУ) Физико-технический институт (ФТИ) Лаборатория радиационного контроля № 31 (Лаборатория РК № 31)
Other Authors:	Prokofjeva M. M. Mariya Mikhaylovna, Proshkina G. M. Galina Mikhaylovna, Lebedev T. D. Timofey Dmitrievich, Shulgin A. A. Aleksandr Anatoljevich, Spirin P. B. Pavel Borisovich, Prasolov V. S. Vladimir Sergeevich, Deev S. M. Sergey Mikhaylovich
Summary:	Title screen Gene therapy is a promising method for treating malignant diseases. One of the main problems is target delivery of therapeutic genes. Here we show that lentiviral vector particles pseudotyped with Mus caroli endogenous retrovirus (McERV) envelope protein can be used for selective transduction of PLLP-expressing cells. As a therapeutic gene in McERV-pseudotyped vector particles we used miniSOG encoding the cytotoxic FMN-binding protein, which can generate reactive oxygen species under illumination. Significant cytotoxic effect (up to 80% of dead cells in population) was observed in PLLP-expressing cells transduced with McERV-pseudotyped vector particles and subjected to illumination. We demonstrated that the McERV-pseudotyped HIV-1 based lentiviral vector particles are an effective tool for selective photoinduced destruction of PLLP-expressing cells. Режим доступа: по договору с организацией-держателем ресурса
Published:	2017
Subjects:	электронный ресурс труды учёных ТПУ доставка генов лентивирусы фотоиндуцированные процессы цитотоксичность фотоиндуцированная цитотоксичность gene delivery lentiviral vectors miniSOG plasmolipin photoinduced cytotoxicity blue light illumination
Online Access:	https://doi.org/10.1016/j.biochi.2017.09.004
Format:	Electronic Book Chapter
KOHA link:	https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=657691

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