Lentiviral gene delivery to plasmolipin-expressing cells using Mus caroli endogenous retrovirus envelope protein; Biochimie; Vol. 142
| Parent link: | Biochimie Vol. 142.— 2017.— [P. 226-233] |
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| Körperschaft: | |
| Weitere Verfasser: | , , , , , , |
| Zusammenfassung: | Title screen Gene therapy is a promising method for treating malignant diseases. One of the main problems is target delivery of therapeutic genes. Here we show that lentiviral vector particles pseudotyped with Mus caroli endogenous retrovirus (McERV) envelope protein can be used for selective transduction of PLLP-expressing cells. As a therapeutic gene in McERV-pseudotyped vector particles we used miniSOG encoding the cytotoxic FMN-binding protein, which can generate reactive oxygen species under illumination. Significant cytotoxic effect (up to 80% of dead cells in population) was observed in PLLP-expressing cells transduced with McERV-pseudotyped vector particles and subjected to illumination. We demonstrated that the McERV-pseudotyped HIV-1 based lentiviral vector particles are an effective tool for selective photoinduced destruction of PLLP-expressing cells. Режим доступа: по договору с организацией-держателем ресурса |
| Sprache: | Englisch |
| Veröffentlicht: |
2017
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| Schlagworte: | |
| Online-Zugang: | https://doi.org/10.1016/j.biochi.2017.09.004 |
| Format: | Elektronisch Buchkapitel |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=657691 |
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| 200 | 1 | |a Lentiviral gene delivery to plasmolipin-expressing cells using Mus caroli endogenous retrovirus envelope protein |f M. M. Prokofjeva [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: 25 tit.] | ||
| 330 | |a Gene therapy is a promising method for treating malignant diseases. One of the main problems is target delivery of therapeutic genes. Here we show that lentiviral vector particles pseudotyped with Mus caroli endogenous retrovirus (McERV) envelope protein can be used for selective transduction of PLLP-expressing cells. As a therapeutic gene in McERV-pseudotyped vector particles we used miniSOG encoding the cytotoxic FMN-binding protein, which can generate reactive oxygen species under illumination. Significant cytotoxic effect (up to 80% of dead cells in population) was observed in PLLP-expressing cells transduced with McERV-pseudotyped vector particles and subjected to illumination. We demonstrated that the McERV-pseudotyped HIV-1 based lentiviral vector particles are an effective tool for selective photoinduced destruction of PLLP-expressing cells. | ||
| 333 | |a Режим доступа: по договору с организацией-держателем ресурса | ||
| 461 | |t Biochimie | ||
| 463 | |t Vol. 142 |v [P. 226-233] |d 2017 | ||
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a труды учёных ТПУ | |
| 610 | 1 | |a доставка генов | |
| 610 | 1 | |a лентивирусы | |
| 610 | 1 | |a фотоиндуцированные процессы | |
| 610 | 1 | |a цитотоксичность | |
| 610 | 1 | |a фотоиндуцированная цитотоксичность | |
| 610 | 1 | |a gene delivery | |
| 610 | 1 | |a lentiviral vectors | |
| 610 | 1 | |a miniSOG | |
| 610 | 1 | |a plasmolipin | |
| 610 | 1 | |a photoinduced cytotoxicity | |
| 610 | 1 | |a blue light illumination | |
| 701 | 1 | |a Prokofjeva |b M. M. |g Mariya Mikhaylovna | |
| 701 | 1 | |a Proshkina |b G. M. |g Galina Mikhaylovna | |
| 701 | 1 | |a Lebedev |b T. D. |g Timofey Dmitrievich | |
| 701 | 1 | |a Shulgin |b A. A. |g Aleksandr Anatoljevich | |
| 701 | 1 | |a Spirin |b P. B. |g Pavel Borisovich | |
| 701 | 1 | |a Prasolov |b V. S. |g Vladimir Sergeevich | |
| 701 | 1 | |a Deev |b S. M. |c biologist |c the expert of Tomsk Polytechnic University, doctor of biological Sciences |g Sergey Mikhaylovich |3 (RuTPU)RU\TPU\pers\39299 | |
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