Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages
| Parent link: | Materials and Design.— , 1978- Vol. 127.— 2017.— [P. 261-271] |
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| Drugi avtorji: | , , , , , , , , , , , , , , |
| Izvleček: | Title screen Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface. Режим доступа: по договору с организацией-держателем ресурса |
| Jezik: | angleščina |
| Izdano: |
2017
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| Teme: | |
| Online dostop: | https://doi.org/10.1016/j.matdes.2017.04.079 |
| Format: | Elektronski Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=656731 |
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| 200 | 1 | |a Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages |f V. L. Kudryavtseva, K. S. Stankevich, A. Gudima [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: p. 270-271 (80 tit.)] | ||
| 330 | |a Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface. | ||
| 333 | |a Режим доступа: по договору с организацией-держателем ресурса | ||
| 461 | |t Materials and Design |d 1978- | ||
| 463 | |t Vol. 127 |v [P. 261-271] |d 2017 | ||
| 610 | 1 | |a электронный ресурс | |
| 610 | 1 | |a труды учёных ТПУ | |
| 610 | 1 | |a polylactic acid (PLA) | |
| 610 | 1 | |a hyaluronic acid (HA) | |
| 610 | 1 | |a tissue engineering scaffold (TES) | |
| 610 | 1 | |a plasma | |
| 610 | 1 | |a macrophages | |
| 610 | 1 | |a cytokines | |
| 610 | 1 | |a полимолочная кислота | |
| 610 | 1 | |a гиалуроновая кислота | |
| 610 | 1 | |a плазма | |
| 610 | 1 | |a макрофаги | |
| 610 | 1 | |a цитокины | |
| 701 | 1 | |a Kudryavtseva |b V. L. |c physicist |c Engineer of Tomsk Polytechnic University |f 1993- |g Valeriya Lvovna |3 (RuTPU)RU\TPU\pers\38564 |9 20822 | |
| 701 | 1 | |a Stankevich |b K. S. |c Physicist |c Engineer Tomsk Polytechnic University |f 1992- |g Ksenia Sergeevna |3 (RuTPU)RU\TPU\pers\37546 |9 20415 | |
| 701 | 1 | |a Gudima |b A. |g Aleksandru | |
| 701 | 1 | |a Kibler |b E. V. |c specialist in the field of nuclear technologies |c Engineer of Tomsk Polytechnic University |f 1995- |g Elina Vitaljevna |3 (RuTPU)RU\TPU\pers\46672 |9 22328 | |
| 701 | 1 | |a Zhukov |b Yu. N. |g Yury Nikolaevich | |
| 701 | 1 | |a Bolbasov |b E. N. |c physicist |c Senior Researcher at Tomsk Polytechnic University, Candidate of Technical Sciences |f 1981- |g Evgeny Nikolaevich |3 (RuTPU)RU\TPU\pers\30857 |9 15103 | |
| 701 | 1 | |a Malashicheva |b A. B. |g Anna Borisovna | |
| 701 | 1 | |a Zhuravlev |b M. V. |c physicist |c Researcher of Tomsk Polytechnic University, Candidate of Technical Sciences |f 1986- |g Mikhail Valerievich |y Tomsk |3 (RuTPU)RU\TPU\pers\34637 |9 17999 | |
| 701 | 1 | |a Ryabov |b V. B. |g Vladimir Borisovich | |
| 701 | 1 | |a Liu |b T. |g Tengfei | |
| 701 | 1 | |a Filimonov |b V. D. |c Russian chemist |c Professor of the TPU |f 1945- |g Viktor Dmitrievich |3 (RuTPU)RU\TPU\pers\26423 |9 12127 | |
| 701 | 1 | |a Remnev (Remnyov) |b G. E. |c physicist |c Professor of Tomsk Polytechnic University, Doctor of technical sciences |f 1948- |g Gennady Efimovich |3 (RuTPU)RU\TPU\pers\31500 |9 15661 | |
| 701 | 1 | |a Kluter |b H. |g Haralg | |
| 701 | 1 | |a Kzhyshkowska |b Ju. |g Julia | |
| 701 | 1 | |a Tverdokhlebov |b S. I. |c physicist |c Associate Professor of Tomsk Polytechnic University, Candidate of physical and mathematical science |f 1961- |g Sergei Ivanovich |3 (RuTPU)RU\TPU\pers\30855 |9 15101 | |
| 801 | 2 | |a RU |b 63413507 |c 20201119 |g RCR | |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.matdes.2017.04.079 |z https://doi.org/10.1016/j.matdes.2017.04.079 |
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