Spheroids of HER2-Positive Breast Adenocarcinoma for Studying Anticancer Immunotoxins In Vitro; Acta Naturae; Vol. 9, №1 (32)

Spheroids of HER2-Positive Breast Adenocarcinoma for Studying Anticancer Immunotoxins In Vitro; Acta Naturae; Vol. 9, №1 (32)

Bibliographic Details
Parent link:Acta Naturae
Vol. 9, №1 (32).— 2017.— [P. 38-43]
Corporate Author: Национальный исследовательский Томский политехнический университет (ТПУ) Физико-технический институт (ФТИ) Лаборатория радиационного контроля № 31 (Лаборатория РК № 31)
Other Authors: Balalaeva I. V. Irina Vladimirovna, Sokolova E. A. Evgeniya Anatoljevna, Puzhikhina A. D. Alena Dmitrievna, Brilkina A. A. Anna Aleksandrovna, Deev S. M. Sergey Mikhaylovich
Summary:Title screen
Tumor response to therapeutic treatment is largely determined by its heterogeneity and the presence of intercellular junctions, hindering the penetration of large molecules deep into the three-dimensional structure of the tumor. In that context, 3D in vitro tumor models such as cancer cell spheroids are becoming increasingly popular. We obtained spheroids of human breast adenocarcinoma SKBR-3 overexpressing the HER2 cancer marker. The toxicity of HER2-targeted immunotoxin 4D5scFv-PE40 against spheroids was shown to be several orders of magnitude lower compared to a monolayer cell culture. The significant difference in the severity of the immunotoxin effect can be explained by the fact that it ineffectively penetrates the spheroid and predominantly influences the cells of the outer layers. The resulting tumor spheroid model can be used in development of drugs for targeted therapy as well as to study ways to improve the efficiency of anticancer agents by targeting cell-cell contacts.
Language:English
Published: 2017
Subjects:
электронный ресурс
труды учёных ТПУ
опухоли
сфероиды
3D-модели
соединения
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406658/
Format: Electronic Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=655503

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