Spheroids of HER2-Positive Breast Adenocarcinoma for Studying Anticancer Immunotoxins In Vitro

Spheroids of HER2-Positive Breast Adenocarcinoma for Studying Anticancer Immunotoxins In Vitro

Detalhes bibliográficos
Parent link:Acta Naturae
Vol. 9, №1 (32).— 2017.— [P. 38-43]
Autor Corporativo: Национальный исследовательский Томский политехнический университет (ТПУ) Физико-технический институт (ФТИ) Лаборатория радиационного контроля № 31 (Лаборатория РК № 31)
Outros Autores: Balalaeva I. V. Irina Vladimirovna, Sokolova E. A. Evgeniya Anatoljevna, Puzhikhina A. D. Alena Dmitrievna, Brilkina A. A. Anna Aleksandrovna, Deev S. M. Sergey Mikhaylovich
Resumo:Title screen
Tumor response to therapeutic treatment is largely determined by its heterogeneity and the presence of intercellular junctions, hindering the penetration of large molecules deep into the three-dimensional structure of the tumor. In that context, 3D in vitro tumor models such as cancer cell spheroids are becoming increasingly popular. We obtained spheroids of human breast adenocarcinoma SKBR-3 overexpressing the HER2 cancer marker. The toxicity of HER2-targeted immunotoxin 4D5scFv-PE40 against spheroids was shown to be several orders of magnitude lower compared to a monolayer cell culture. The significant difference in the severity of the immunotoxin effect can be explained by the fact that it ineffectively penetrates the spheroid and predominantly influences the cells of the outer layers. The resulting tumor spheroid model can be used in development of drugs for targeted therapy as well as to study ways to improve the efficiency of anticancer agents by targeting cell-cell contacts.
Idioma:inglês
Publicado em: 2017
Assuntos:
электронный ресурс
труды учёных ТПУ
опухоли
сфероиды
3D-модели
соединения
Acesso em linha:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406658/
Formato: Recurso Electrónico Capítulo de Livro
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=655503

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