Morphofunctional changes of Jurkat T lymphoblasts upon short-term contact with a relief calcium phosphate surface
| Parent link: | Cell and Tissue Biology Vol. 11, iss. 1.— 2017.— [P. 59–64] |
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| Ente Autore: | |
| Altri autori: | , , , , , , , |
| Riassunto: | Title screen Human leukemic T lymphoblastoid cells (hereinafter, Jurkat T cells) have been used to model the morphofunctional reaction of T lymphocytes to 24-h in vitro contact with relief (roughness index Ra = 2.2-2.7 μm) pure titanium substrates (12 × 12 × 1 mm3) covered by a calcium phosphate (CP) bilateral coating prepared by the microarc method. Jurkat T cells placed in plastic multiwall plates (2D control of culture growth), as well as cells in contact for 24 h with oxide (TiO2) microarc coating on pure titanium substrate (3D control), served as comparison tests. From 27 to 98% of immortalized cells in the 2D control culture had the CD3+CD4+CD71+CD45RA+ immunophenotype and secreted IL-2, IL-4, IL-8, IL-10, and TNFα, but not IL-1b and IL-6. Other markers of cell activation, differentiation, maturation, and death (CD8, CD16, CD56, CD25, and CD95) were found in 0-2.5% of cells. IL-8 release elevated by the microtextured CP surface by 183 and 160% relative to 2D- and 3D-control cultures, respectively. The CD4/CD8 ratio dropped to 9: 1 (13: 1 and 82: 1 in 2D and 3D controls, respectively) due to CD4+ cell depletion and an increase in CD8+ cell number. The total cell number (TCN) in Jurkat T cell culture after 24-h contact with CP coating was decreased to 88% compared to 2D control (p < 0.04), which indicated cell division suppression. A drop in total cell number was accompanied with accelerated IL-8 secretion (r = −0.97, p < 0.00009). Low concentrations of IL-8 (pg/mL) induced apoptosis (r = 0.94, p < 0.0001). These results show that CP, rather than TiO2, coating is a preferential surface material for endoprosthesis replacement and fracture osteosynthesis in patients suffering from hematological and bone malignancies. Режим доступа: по договору с организацией-держателем ресурса |
| Lingua: | inglese |
| Pubblicazione: |
2017
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| Soggetti: | |
| Accesso online: | http://dx.doi.org/10.1134/S1990519X17010060 |
| Natura: | Elettronico Capitolo di libro |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=654697 |
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| 200 | 1 | |a Morphofunctional changes of Jurkat T lymphoblasts upon short-term contact with a relief calcium phosphate surface |f I. A. Khlusov [et al.] | |
| 203 | |a Text |c electronic | ||
| 300 | |a Title screen | ||
| 320 | |a [References: p. 63-64 (34 tit.)] | ||
| 330 | |a Human leukemic T lymphoblastoid cells (hereinafter, Jurkat T cells) have been used to model the morphofunctional reaction of T lymphocytes to 24-h in vitro contact with relief (roughness index Ra = 2.2-2.7 μm) pure titanium substrates (12 × 12 × 1 mm3) covered by a calcium phosphate (CP) bilateral coating prepared by the microarc method. Jurkat T cells placed in plastic multiwall plates (2D control of culture growth), as well as cells in contact for 24 h with oxide (TiO2) microarc coating on pure titanium substrate (3D control), served as comparison tests. From 27 to 98% of immortalized cells in the 2D control culture had the CD3+CD4+CD71+CD45RA+ immunophenotype and secreted IL-2, IL-4, IL-8, IL-10, and TNFα, but not IL-1b and IL-6. Other markers of cell activation, differentiation, maturation, and death (CD8, CD16, CD56, CD25, and CD95) were found in 0-2.5% of cells. IL-8 release elevated by the microtextured CP surface by 183 and 160% relative to 2D- and 3D-control cultures, respectively. The CD4/CD8 ratio dropped to 9: 1 (13: 1 and 82: 1 in 2D and 3D controls, respectively) due to CD4+ cell depletion and an increase in CD8+ cell number. The total cell number (TCN) in Jurkat T cell culture after 24-h contact with CP coating was decreased to 88% compared to 2D control (p < 0.04), which indicated cell division suppression. A drop in total cell number was accompanied with accelerated IL-8 secretion (r = −0.97, p < 0.00009). Low concentrations of IL-8 (pg/mL) induced apoptosis (r = 0.94, p < 0.0001). These results show that CP, rather than TiO2, coating is a preferential surface material for endoprosthesis replacement and fracture osteosynthesis in patients suffering from hematological and bone malignancies. | ||
| 333 | |a Режим доступа: по договору с организацией-держателем ресурса | ||
| 461 | |t Cell and Tissue Biology | ||
| 463 | |t Vol. 11, iss. 1 |v [P. 59–64] |d 2017 | ||
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| 610 | 1 | |a цитокины | |
| 610 | 1 | |a апотоз | |
| 610 | 1 | |a некрозы | |
| 610 | 1 | |a фосфат кальция | |
| 610 | 1 | |a микротекстура | |
| 610 | 1 | |a jurkat T-cell line | |
| 610 | 1 | |a membrane markers | |
| 610 | 1 | |a cytokines | |
| 610 | 1 | |a apoptosis | |
| 610 | 1 | |a necrosis | |
| 610 | 1 | |a titanium substrates | |
| 610 | 1 | |a microarc calcium phosphate coating | |
| 610 | 1 | |a microtexture | |
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