Immunological and phenotypic characterization of cell constituents of breast milk; Cell and Tissue Biology; Vol. 10, iss. 5

Immunological and phenotypic characterization of cell constituents of breast milk; Cell and Tissue Biology; Vol. 10, iss. 5

Bibliographic Details
Parent link:Cell and Tissue Biology
Vol. 10, iss. 5.— 2016.— [P. 410-415]
Corporate Author: Национальный исследовательский Томский политехнический университет Физико-технический институт Кафедра экспериментальной физики
Other Authors: Zaytsev K. V. Konstantin Vasiljevich, Mezheritsky S. A. Stanislav Aleksandrovich, Stepanenko N. P. Nina Petrovna, Gostyukhina A. A. Alena Anatoljevna, Zhukova O. B. Oksana Borisovna, Kondratjeva E. I. Elena Ivanovna, Stepanov I. A. Igor Ardalionovich, Dzyuman A. N. Anna Nikolaevna, Nikolaevskaya E. E. Ekaterina Evgenjevna, Vorobjev V. A. Viktor Aleksandrovich, Abdulkina N. G. Nataljya Gennadjevna, Zaytsev A. A. Aleksey Aleksandrovich, Yurjev S. Yu. Sergey Yurjevich, Korshunova O. P. Olga Petrovna, Litvinova L. S. Larisa Sergeevna, Khlusov I. A. Igor Albertovich
Summary:Title screen
The morphology and immunological phenotype of plastic-adherent cells from human colostrum were studied in an in vitro short-term culture (6–7 days) using antibodies to CD3, CD31, CD34, CD45, CD68, vimentin, and osteocalcin. In 20% of the analyzed cultural flasks, nearly equal proportion of fibroblast-like cells and rounded cells was observed. In the 80% of the flasks, cells of regular shape were detected with the presence of single fibroblasts. The diameter of flattened cells ranged from 10 to 100 ?m. All plastic-adherent cells did not express CD3 and showed weak binding (w) to the antibodies against CD31, CD34, and CD45. At the same time, we identified adherent cells that readily bound the antibodies against CD68, vimentin, and osteocalcin. According to the literature data, the CD68 + CD3–CD31wCD34wCD45w immunological phenotype of the majority of the adherent cells from the colostrum allows them to be classified as monocytes/macrophages. High expression of stromal antigens—vimentin and osteocalcin—in 40–45% of the adherent cells in the culturing medium lacking any osteogenic supplements (?-glycerophosphate, ascorbic acid, and dexamethason) implies the presence of osteoblasts in the colostrum, which differentiate from mesenchymal stem cells under the action of humoral factors contained in breast milk.
Режим доступа: по договору с организацией-держателем ресурса
Language:English
Published: 2016
Subjects:
электронный ресурс
труды учёных ТПУ
иммунологические исследования
грудное молоко
Online Access:http://dx.doi.org/10.1134/S1990519X1605014X
Format: Electronic Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=652135

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