Pilot in vitro study of the parameters of artificial niche for osteogenic differentiation of human stromal stem cell pool; Bulletin of Experimental Biology and Medicine; Vol. 150, iss. 4

Pilot in vitro study of the parameters of artificial niche for osteogenic differentiation of human stromal stem cell pool; Bulletin of Experimental Biology and Medicine; Vol. 150, iss. 4

Bibliographic Details
Parent link:Bulletin of Experimental Biology and Medicine: Scientific Journal
Vol. 150, iss. 4.— 2011.— [P. 535-542]
Other Authors: Khlusov I. A. Igor Albertovich, Khlusova M. Yu., Zaitsev K. V., Kolokol’tsova T. D., Sharkeev Yu. P. Yury Petrovich, Pichugin V. F. Vladimir Fyodorovich, Легостаева Е. В. Елена Викторовна, Trofimova I. E., Klimov A. S., Zhdanova A. I.
Summary:Title screen
The aim of this research is experimental investigation of the topography and evaluation of some parameters of artificial microterritories promoting osteogenic differentiation of stromal stem cells. A technique of short-term culturing of prenatal human lung stromal cells with fibroblastoid morphology on calcium phosphate substrates with known topography was used. Judging from secretory activity of the cell culture (osteocalcin, alkaline phosphatase), stromal stem cells directly interacting with calcium phosphate discs have advantage in manifestation of osteoblast-like functional activity in comparison with cells cultured on plastic. Rough surfaces of calcium phosphate discs stimulate the formation of spatial human fibroblastoid cell culture. The cells with positive reaction to acid phosphatase are located on spheroliths forming the relief of calcium phosphate coatings. The cells with positive reaction to alkaline phosphatase (marker of osteoblasts) populate hollows (niches) of the artifi cial surface. The niche for induction of osteogenic differentiation of human multipotent mesenchymal stem cells is apparently a structural and functional formation. It can be characterized by an index calculated as the ratio of the total area occupied by alkaline phosphatase-positive cells to the area of artifi cial surface occupied by one stained cell.
Режим доступа: по договору с организацией-держателем ресурса
Language:English
Published: 2011
Subjects:
электронный ресурс
труды учёных ТПУ
Online Access:http://dx.doi.org/10.1007/s10517-011-1184-4
Format: Electronic Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=642689

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