Development of a Method for Producing and Purifying N2-Carboxyethyl-2'-Deoxyguanosine for Molecular-Biological Research; Advanced Materials Research; Vol. 1085 : Prospects of Fundamental Sciences Development (PFSD-2014)

Development of a Method for Producing and Purifying N2-Carboxyethyl-2'-Deoxyguanosine for Molecular-Biological Research; Advanced Materials Research; Vol. 1085 : Prospects of Fundamental Sciences Development (PFSD-2014)

Bibliographic Details
Parent link:Advanced Materials Research: Scientific Journal
Vol. 1085 : Prospects of Fundamental Sciences Development (PFSD-2014).— 2015.— [P. 436-440]
Corporate Author: Национальный исследовательский Томский политехнический университет (ТПУ) Институт природных ресурсов (ИПР) Кафедра технологии органических веществ и полимерных материалов (ТОВПМ)
Other Authors: Krivoshchekov S. V. Sergey Vladimirovich, Bogdanov A. O., Ogorodova L. M., Dementeva N. B., Saltykova I. V., Marchenko R. D., Ivanov V. V., Mayboroda O. A., Sazonov A. E.
Summary:Title screen
Methylglyoxal (MG) – is a high reactive α- oxoaldehyde, which can be synthesized through various biochemical processes in vivo. MG is capable of interacting with nucleophilic groups of proteins, lipids and nucleic acids which leads to their glycation. Covalent compounds that formed in this process were named advanced glycation end products. Advanced glycation end products play an important role in the pathogenesis of diabetes, chronic inflammation, cancer and Alzheimer's disease. One of the most stable compounds, which is formed by reacting of methylglyoxal with the nitrogenous bases of the DNA molecule is N2- carboxyethyl -2' - deoxyguanosine (CEdG). This compound can be used as a marker for monitoring of various diseases, investigation of the role of glycation end products in their pathogenesis, as well as to search for the therapeutic targets. To perform our molecular-biological investigations by mass spectrometry, we synthesized N2- carboxyethyl -2' - deoxyguanosine, and have proposed a new method for purifying compounds by reverse - phase HPLC. As a result, we achieved the optimal purity of the sample, while substantially reducing the cost of the purification procedure.
Режим доступа: по договору с организацией-держателем ресурса
Language:English
Published: 2015
Series:Material Science in Biology and Medicine
Subjects:
электронный ресурс
труды учёных ТПУ
масс-спектрометрия
метилглиоксаль
биологические исследования
Online Access:http://dx.doi.org/10.4028/www.scientific.net/AMR.1085.436
Format: Electronic Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=641252

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