Electrochemical Determination of Heparin in Pharmaceuticals with Using Malachite Green; Advanced Materials Research; Vol. 1040 : High Technology: Research and Applications 2014 (HTRA 2014)

Λεπτομέρειες βιβλιογραφικής εγγραφής
Parent link:Advanced Materials Research: Scientific Journal
Vol. 1040 : High Technology: Research and Applications 2014 (HTRA 2014).— 2014.— [P. 292-296]
Κύριος συγγραφέας: Vishenkova D. A. Dariya Aleksandrovna
Συγγραφή απο Οργανισμό/Αρχή: Национальный исследовательский Томский политехнический университет (ТПУ) Институт природных ресурсов (ИПР) Кафедра физической и аналитической химии (ФАХ)
Άλλοι συγγραφείς: Korotkova E. I. Elena Ivanovna, Dorozhko E. V. Elena Vladimirovna
Περίληψη:Title screen
In this paper for determination of heparin in pharmaceuticals by constant-current sweep voltammetry on the mercury-film electrode (MFE) we used cationic malachite green (MG) dye which is capable to form a complex with heparin. In the 0.05 M potassium tetraoxalate (KH[3]C[4]O[8]∙2H[2]O) buffer solution with pH 1.65 MG had a well-defined peak of electroreduction at -0.46 V (vs. CSE). During the study of electrochemical properties of heparin in complex with MG in cathodic potential range from 0.0 V to -1.3 V on MFE besides the signal from the dye at E=-0.46 V the second signal at E=-0.87 V was found. Increasing the concentration of heparin in the solution led to decrease of signal intensity at E=-0.87 V. Optimum conditions for the electrochemical determination of heparin in dosage form with using MG were picked up. Influence of auxiliary components of heparin pharmacological solution on electroreduction of the complex MG-Hep was investigated. Under optimal conditions the decrease of reduction peak currents at E=-0.87 V was proportional to heparin concentration in the range from 3.9∙10{-4} mg/ml to 3.9∙10{-3} mg/ml.
Режим доступа: по договору с организацией-держателем ресурса
Γλώσσα:Αγγλικά
Έκδοση: 2014
Σειρά:Chemical Technologies
Θέματα:
Διαθέσιμο Online:http://dx.doi.org/10.4028/www.scientific.net/AMR.1040.292
Μορφή: Ηλεκτρονική πηγή Κεφάλαιο βιβλίου
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=640956

MARC

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330 |a In this paper for determination of heparin in pharmaceuticals by constant-current sweep voltammetry on the mercury-film electrode (MFE) we used cationic malachite green (MG) dye which is capable to form a complex with heparin. In the 0.05 M potassium tetraoxalate (KH[3]C[4]O[8]∙2H[2]O) buffer solution with pH 1.65 MG had a well-defined peak of electroreduction at -0.46 V (vs. CSE). During the study of electrochemical properties of heparin in complex with MG in cathodic potential range from 0.0 V to -1.3 V on MFE besides the signal from the dye at E=-0.46 V the second signal at E=-0.87 V was found. Increasing the concentration of heparin in the solution led to decrease of signal intensity at E=-0.87 V. Optimum conditions for the electrochemical determination of heparin in dosage form with using MG were picked up. Influence of auxiliary components of heparin pharmacological solution on electroreduction of the complex MG-Hep was investigated. Under optimal conditions the decrease of reduction peak currents at E=-0.87 V was proportional to heparin concentration in the range from 3.9∙10{-4} mg/ml to 3.9∙10{-3} mg/ml. 
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