Cell-volume-dependent vascular smooth muscle contraction: role of Na+, K+, 2Cl- cotransport, intracellular Cl- and L-type Ca2+ channels; Pflugers Archiv; Vol. 449, iss. 1

Cell-volume-dependent vascular smooth muscle contraction: role of Na+, K+, 2Cl- cotransport, intracellular Cl- and L-type Ca2+ channels; Pflugers Archiv; Vol. 449, iss. 1

Bibliografske podrobnosti
Parent link:Pflugers Archiv: Scientific Journal
Vol. 449, iss. 1.— 2004.— [P. 42-55]
Drugi avtorji: Anfinogenova Ya. J. Yana Jonovna, Baskakov M. B. Mikhail Borisovich, Kovalev I. V., Kilin A. A., Dulin N. O., Orlov S. N.
Izvleček:Title screen
This study elucidates the role of cell volume in contractions of endothelium-denuded vascular smooth muscle rings (VSMR) from the rat aorta. We observed that hyposmotic swelling as well as hyper- and isosmotic shrinkage led to VSMR contractions. Swelling-induced contractions were accompanied by activation of Ca2+ influx and were abolished by nifedipine and verapamil. In contrast, contractions of shrunken cells were insensitive to the presence of L-type channel inhibitors and occurred in the absence of Ca2+ o. Thirty minutes preincubation with bumetanide, a potent Na+,K+,Cl- cotransport (NKCC) inhibitor, decreased Cl- i content, nifedipine-sensitive 45Ca uptake and contractions triggered by modest depolarization ([K+]o=36 mM). Elevation of [K+]o to 66 mM completely abolished the effect of bumetanide on these parameters. Bumetanide almost completely abrogated phenylephrine-induced contraction, partially suppressed contractions triggered by hyperosmotic shrinkage, but potentiated contractions of isosmotically shrunken VSMR. Our results suggest that bumetanide suppresses contraction of modestly depolarized cells via NKCC inhibition and Cl- i-mediated membrane hyperpolarization, whereas augmented contraction of isosmotically shrunken VSMR by bumetanide is a consequence of suppression of NKCC-mediated regulatory volume increase. The mechanism of bumetanide inhibition of contraction of phenylephrine-treated and hyperosmotically shrunken VSMR should be examined further.
Режим доступа: по договору с организацией-держателем ресурса
Jezik:angleščina
Izdano: 2004
Teme:
электронный ресурс
труды учёных ТПУ
Ca2+ channels
Cell volume
Contraction
Intracellular Cl-
Smooth muscle
Online dostop:http://link.springer.com/article/10.1007/s00424-004-1316-z
Format: Elektronski Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=639203

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