Dissociation of Crk-associated substrate from the vimentin network is regulated by p21-activated kinase on ACh activation of airway smooth muscle; American Journal of Physiology - Lung Cellular and Molecular Physiology Published; Vol. 292, № 1

Dissociation of Crk-associated substrate from the vimentin network is regulated by p21-activated kinase on ACh activation of airway smooth muscle; American Journal of Physiology - Lung Cellular and Molecular Physiology Published; Vol. 292, № 1

গ্রন্থ-পঞ্জীর বিবরন
Parent link:American Journal of Physiology - Lung Cellular and Molecular Physiology Published: Scientific Journal.— , 1997-
Vol. 292, № 1.— 2007.— [P. 240-248]
অন্যান্য লেখক: Ruping Wang, Qing-Fen Li, Anfinogenova Ya. J. Yana Jonovna, Tang D. D.
সংক্ষিপ্ত:Title screen
The intermediate filament protein vimentin has been shown to be required for smooth muscle contraction. The adapter protein p130 Crk-associated substrate (CAS) participates in the signaling processes that regulate force development in smooth muscle. However, the interaction of vimentin filaments with CAS has not been well elucidated. In the present study, ACh stimulation of tracheal smooth muscle strips increased the ratio of soluble to insoluble vimentin (an index of vimentin disassembly) in association with force development. ACh activation also induced vimentin phosphorylation at Ser56 as assessed by immunoblot analysis. More importantly, CAS was found in the cytoskeletal vimentin fraction, and the amount of CAS in cytoskeletal vimentin was reduced in smooth muscle strips on contractile stimulation. CAS redistributed from the myoplasm to the periphery during ACh activation of smooth muscle cells. The ACh-elicited decrease in CAS distribution in cytoskeletal vimentin was attenuated by the downregulation of p21-activated kinase (PAK) 1 with antisense oligodeoxynucleotides. Vimentin phosphorylation at this residue, the ratio of soluble to insoluble vimentin, and active force in smooth muscle strips induced by ACh were also reduced in PAK-depleted tissues. These results suggest that PAK may regulate CAS release from the vimentin intermediate filaments by mediating vimentin phosphorylation at Ser56 and the transition of cytoskeletal vimentin to soluble vimentin. The PAK-mediated dissociation of CAS from the vimentin network may participate in the cellular processes that affect active force development during ACh activation of tracheal smooth muscle tissues.
ভাষা:ইংরেজি
প্রকাশিত: 2007
বিষয়গুলি:
электронный ресурс
труды учёных ТПУ
অনলাইন ব্যবহার করুন:http://ajplung.physiology.org/content/292/1/L240
বিন্যাস: বৈদ্যুতিক গ্রন্থের অধ্যায়
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=639145

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