High detection sensitivity achieved with cryogenic detectors in combination with matrix-assisted laser desorption / ionisation time-of-flight mass spectrometry (MALDI-TOF MS)

High detection sensitivity achieved with cryogenic detectors in combination with matrix-assisted laser desorption / ionisation time-of-flight mass spectrometry (MALDI-TOF MS)

Dades bibliogràfiques
Parent link:European Journal of Mass Spectrometry: Scientific Journal.— , 1995-
Vol. 10, iss. 4.— 2004.— [P. 469-476]
Altres autors: Christ P., Rutzinger S., Koy C., Seidel W., Uchaykin S. V. Sergey Victorovich, Glocker M. O., Probst F.
Sumari:Title screen
Cryogenic detectors directly measure the impact energy of any impinging particle independent of its velocity. Thus a very high, mass-independent, detection efficiency is expected from their application in TOF-MS. The cryogenic detector applied here is based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series using standard sample preparation procedures shows that the detection limit for insulin (Mr: 5,734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target. Detection limits for rhM-CSF ß (Mr: 49,032) and for polyclonal IgG (Mr: ca. 150,000) in the high femtomole and low picomole range, respectively, were established
Idioma:anglès
Publicat: 2004
Matèries:
электронный ресурс
труды учёных ТПУ
криогенные детекторы
cryogenic detector
масс-спектрометрия
MALDI
Accés en línia:http://www.impublications.com/content/abstract?code=E10_0469
https://publications.mpp.mpg.de/?action=search&mpi=MPP-2003-141
Format: Electrònic Capítol de llibre
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=637165

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