Анализ аберрантно-метилированных последовательностей в составе циркулирующих ДНК крови при раке легкого
| Parent link: | Перспективы развития фундаментальных наук=Prospects of Fundamental Sciences Development: сборник научных трудов XIII Международной конференции студентов, аспирантов и молодых ученых, г. Томск, 26-29 апреля 2016 г./ Национальный исследовательский Томский политехнический университет (ТПУ) ; под ред. И. А. Курзиной, Г. А. Вороновой.— , 2016 Т. 4 : Биомедицина.— 2016.— [С. 105-107] |
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| Summary: | Заглавие с титульного экрана Malignant cell transformation is accompanied by two processes of DNA methylation changes: promoter hypermethylation of specific genes and hypomethylation of retrotransposons. The composition of circulating DNA (cirDNA) from plasma and cell-surface-bound circulating DNA (csb-cirDNA) was shown earlier to be altered in the blood of cancer patients due to accumulation of tumor-specific aberrantly methylated DNA fragments, which are currently considered valuable cancer markers. The present study compares LINE-1 retrotransposon methylation patterns in plasma cirDNA and csb-cirDNA from untreated lung cancer patients (LC) and healthy donors. Concentrations of methylated LINE-1 region 1 copies (LINE-1met) were assayed by real-time methylation-specific PCR. In order to normalize the LINE-1 methylation level, the LINE-1 region 2 concentration was evaluated, which was independent of the methylation status (LINE-1Ind). We recorded an statistically significant increase of the LINE-1 methylation index determined as (LINE-1met/LINE-1Ind) due to the profound LINE-1Ind decrease (Mann-Whitney test, p = 0.005). Plasma cirDNA demonstrated no difference in the ratio LINE-1met/LINE-1Ind between LC patients and healthy donors (p = 0.40). The data obtained agree with our earlier results, which showed that csb-cirDNA was a highly informative material for lung cancer diagnostics. |
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2016
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| Online Access: | http://earchive.tpu.ru/handle/11683/25984 |
| Format: | Electronic Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=618079 |
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| 200 | 1 | |a Анализ аберрантно-метилированных последовательностей в составе циркулирующих ДНК крови при раке легкого |d Analysis of aberrantly methylated sequnces in circulating DNA from blood of patients with lung cancer |f А. А. Пономарева, А. А. Бондарь, А. Ю. Добродеев |g науч. рук. Н. В. Чердынцева | |
| 203 | |a Текст |c электронный | ||
| 230 | |a 1 компьютерный файл (pdf; 188 Кb) | ||
| 300 | |a Заглавие с титульного экрана | ||
| 320 | |a [Библиогр.: с. 107 (8 назв.)] | ||
| 330 | |a Malignant cell transformation is accompanied by two processes of DNA methylation changes: promoter hypermethylation of specific genes and hypomethylation of retrotransposons. The composition of circulating DNA (cirDNA) from plasma and cell-surface-bound circulating DNA (csb-cirDNA) was shown earlier to be altered in the blood of cancer patients due to accumulation of tumor-specific aberrantly methylated DNA fragments, which are currently considered valuable cancer markers. The present study compares LINE-1 retrotransposon methylation patterns in plasma cirDNA and csb-cirDNA from untreated lung cancer patients (LC) and healthy donors. Concentrations of methylated LINE-1 region 1 copies (LINE-1met) were assayed by real-time methylation-specific PCR. In order to normalize the LINE-1 methylation level, the LINE-1 region 2 concentration was evaluated, which was independent of the methylation status (LINE-1Ind). We recorded an statistically significant increase of the LINE-1 methylation index determined as (LINE-1met/LINE-1Ind) due to the profound LINE-1Ind decrease (Mann-Whitney test, p = 0.005). Plasma cirDNA demonstrated no difference in the ratio LINE-1met/LINE-1Ind between LC patients and healthy donors (p = 0.40). The data obtained agree with our earlier results, which showed that csb-cirDNA was a highly informative material for lung cancer diagnostics. | ||
| 461 | 1 | |0 (RuTPU)RU\TPU\conf\16353 |t Перспективы развития фундаментальных наук |l Prospects of Fundamental Sciences Development |o сборник научных трудов XIII Международной конференции студентов, аспирантов и молодых ученых, г. Томск, 26-29 апреля 2016 г. |o в 7 т. |f Национальный исследовательский Томский политехнический университет (ТПУ) ; под ред. И. А. Курзиной, Г. А. Вороновой |d 2016 | |
| 463 | 0 | |0 (RuTPU)RU\TPU\conf\16357 |t Т. 4 : Биомедицина |v [С. 105-107] |d 2016 | |
| 510 | 1 | |a Analysis of aberrantly methylated sequnces in circulating DNA from blood of patients with lung cancer |z eng | |
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| 702 | 1 | |a Чердынцева |b Н. В. |4 727 | |
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