Анализ аберрантно-метилированных последовательностей в составе циркулирующих ДНК крови при раке легкого
| Parent link: | Перспективы развития фундаментальных наук=Prospects of Fundamental Sciences Development: сборник научных трудов XIII Международной конференции студентов, аспирантов и молодых ученых, г. Томск, 26-29 апреля 2016 г./ Национальный исследовательский Томский политехнический университет (ТПУ) ; под ред. И. А. Курзиной, Г. А. Вороновой.— , 2016 Т. 4 : Биомедицина.— 2016.— [С. 105-107] |
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| Summary: | Заглавие с титульного экрана Malignant cell transformation is accompanied by two processes of DNA methylation changes: promoter hypermethylation of specific genes and hypomethylation of retrotransposons. The composition of circulating DNA (cirDNA) from plasma and cell-surface-bound circulating DNA (csb-cirDNA) was shown earlier to be altered in the blood of cancer patients due to accumulation of tumor-specific aberrantly methylated DNA fragments, which are currently considered valuable cancer markers. The present study compares LINE-1 retrotransposon methylation patterns in plasma cirDNA and csb-cirDNA from untreated lung cancer patients (LC) and healthy donors. Concentrations of methylated LINE-1 region 1 copies (LINE-1met) were assayed by real-time methylation-specific PCR. In order to normalize the LINE-1 methylation level, the LINE-1 region 2 concentration was evaluated, which was independent of the methylation status (LINE-1Ind). We recorded an statistically significant increase of the LINE-1 methylation index determined as (LINE-1met/LINE-1Ind) due to the profound LINE-1Ind decrease (Mann-Whitney test, p = 0.005). Plasma cirDNA demonstrated no difference in the ratio LINE-1met/LINE-1Ind between LC patients and healthy donors (p = 0.40). The data obtained agree with our earlier results, which showed that csb-cirDNA was a highly informative material for lung cancer diagnostics. |
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2016
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| Online Access: | http://earchive.tpu.ru/handle/11683/25984 |
| Format: | Electronic Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=618079 |
| Summary: | Заглавие с титульного экрана Malignant cell transformation is accompanied by two processes of DNA methylation changes: promoter hypermethylation of specific genes and hypomethylation of retrotransposons. The composition of circulating DNA (cirDNA) from plasma and cell-surface-bound circulating DNA (csb-cirDNA) was shown earlier to be altered in the blood of cancer patients due to accumulation of tumor-specific aberrantly methylated DNA fragments, which are currently considered valuable cancer markers. The present study compares LINE-1 retrotransposon methylation patterns in plasma cirDNA and csb-cirDNA from untreated lung cancer patients (LC) and healthy donors. Concentrations of methylated LINE-1 region 1 copies (LINE-1met) were assayed by real-time methylation-specific PCR. In order to normalize the LINE-1 methylation level, the LINE-1 region 2 concentration was evaluated, which was independent of the methylation status (LINE-1Ind). We recorded an statistically significant increase of the LINE-1 methylation index determined as (LINE-1met/LINE-1Ind) due to the profound LINE-1Ind decrease (Mann-Whitney test, p = 0.005). Plasma cirDNA demonstrated no difference in the ratio LINE-1met/LINE-1Ind between LC patients and healthy donors (p = 0.40). The data obtained agree with our earlier results, which showed that csb-cirDNA was a highly informative material for lung cancer diagnostics. |
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