Reverse micellar partitioning of Bovine Serum Albumin with novel system; Resource-Efficient Technologies; Vol. 3, iss. 4

Reverse micellar partitioning of Bovine Serum Albumin with novel system; Resource-Efficient Technologies; Vol. 3, iss. 4

Бібліографічні деталі
Parent link:Resource-Efficient Technologies: electronic scientific journal/ National Research Tomsk Polytechnic University (TPU).— , 2015-.— 2405-6537
Vol. 3, iss. 4.— 2017.— [P. 491-494]
Автор: Swapnali S. Pawar
Інші автори: Regupathi I., Prasanna B. D.
Резюме:Title screen
To overcome the difficulties associated with the conventional extraction process like poor selective extraction of biomolecule and scale up of the process, the reverse micellar system consist of AOT/n-heptanol was considered to extract Bovine Serum Albumin (BSA) as a model biomolecule. The maximum forward extraction of BSA from aqueous phase to micelle phase was observed at AOT concentration 160 mM, aqueous phase pH value of 4, NaCl concentration 0.8 M and 95% back extraction of BSA from micelle phase to stripping phase was obtained at 1 M NaCl concentration with the pH of 7.5. HPLC analysis confirmed the stability of BSA during extraction. The size and water content of the reverse micelle was also reported. The obtained results emphasize the application of the AOT/n-heptanol reverse micellar system for the extraction of BSA and may be utilized for the selective extraction of similar hydrophilic proteins from the complex sources.
Мова:Англійська
Опубліковано: 2017
Предмети:
труды учёных ТПУ
электронный ресурс
альбумин
экстрация
биомолекулы
концентрация
селективное извлечение
Онлайн доступ:http://earchive.tpu.ru/handle/11683/50333
Формат: Електронний ресурс Частина з книги
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=575716

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